In Vitro And In Vivo Anti-tumor Effects Of CIK Combined With Astragalus Polysaccharides Upon Cervical Cancer Hela Cells

Objective: To research the in vitro and in vivo inhibitory effects of cytokine-induced killer(CIK)cells combined with Astragalus Polysaccharides(APS)from healthy donors against cervical cell line Hela.Methods: CIK cells were generated from peripheral blood mononuclear cells(PBMC)of 10 healthy blood donors in vitro by different cytokinees.These effective cells were identified at different intervals by MTT assays and flow cytometry analysis.And the cytotoxicity of CIK cells were assessed by 51 Cr release assay.Tumor-bearing mice were divided into normal saline control group,ASP group,CIK group and CIK combined with APS group.The anti-tumor activity of each group were evaluated in Balb/c nude mice bearing Hela cervical cancer.Results: The flow cytometry analysis showed that the percentage of CD3+CD56+cells increased significantly in day 14.The cytotoxicity rate of Hela cells in CIK combined with ASP group was slightly higher than those in CIK group(P>0.05)whilst APS show no cytotoxicity directly.In vivo results showed that CIK combined with ASP inhibited the growth of Hela-implanted tumors significantly,and the inhibitory rate was 80.6%.The volume of tumor was smaller than those in other groups.Conclusion: CIK cells combined with Chinese medicine have a stronger suppression on the growth of cervical cancer cells in vivo,providing an experimental basis for CIK clinical application as an adoptive immunotherapy.