Expression And Clinical Significance Of Tumor Antigen NY-ESO-1 Protein In Esophageal Squamous Cell Carcinoma

Esophageal squamous cell carcinoma is one of the most common malignant tumors which threatens human health and the treatment is extremely difficult. There is no other more effective method except surgery for the time being, the mortality rate of esophageal cancer in China is the highest in the world. Recently, with the progress of cellular biology and exploration of tumor ummunization, new tumor-specific antigens constantly found, among which,Cancer-Testis Antigen(CTA) is considered the most promising one. Tumor specific antigen NY-ESO-1 found in recent years belongs to cancer testis antigen family(CTA), which is only expressed in the testis of the highly immunogenic antigen and attracts more and more scholars’ attention.The study have shown that NY-ESO-1 antigen was significantly expressed in a variety of tumor tissues, such as prostate cancer, breast cancer and ovarian cancer. Because of the unique expression and immune characteristics of NY-SEO-1 in tumor tissue, it can play an important role in immune regulation. Therefore, it is very necessary to explore the following questions: Is NY-ESO-1 antigen highly expressed in esophageal squamous cell carcinoma? if so, does it have corelation with each clinical biology of esophageal squamous cell carcinoma? Can it become an ideal immunotherppy genes, thus providing a basis for immunotherapy of esoghageal cancer?The paper uses immunohistochemical method to detect the expression of NY-ESO-1 protein in esophageal squamous cell carcinoma tissues. After compared with the expression of corresponding normal tissue, the corelation of biological indicators for each patient and the clinical significance of this antigen expression can be analyzed, which provides the theory support for the clinical treatment of squamous cell carcinoma of the esophagus. in esophageal squamous cell carcinoma and its clinical significance. Then, it compares with the expression of the corresponding normal esophageal tissues. Finally, it analyzes the relationship between the biological indicators and prognosis. This work will lay the foundation for immunotherapy of esophageal squamous cell carcinoma with NY-ESO-1 antigen. Objective: First, this paper studies the expression of NY-ESO-1 proteinMethods: 78 cases of advanced esophageal squamous cell carcinoma and 10 cases of normal esophageal tissue specimens are selected to establish the tissue microarray. By immunohistochemical two-steps En Vision, this paper detects the expression of NY-ESO-1 protein in esophageal squamous carcinoma tissue and related normal tissue, analyzes relationship between its expression and biological index and its correlation with prognosis. SPSS statistical software was used to analyze the data obtained by Log-rank test and Kaplan-Meier test. The survival curve of NY-ESO-1 was plotted, and the relationship between NY-ESO-1 antigen and cell growth of esophageal squamous cell carcinoma was analyzed.Results: Among the 78 cases of esophageal cancer, 64 cases(82.1%) were positive for NY-ESO-1 expression, 14 cases(17.9%) were negative for NY-ESO-1 expression and NY-ESO-1 protein expression was not found in 10 cases of normal esophageal mucosa.Conclusions: The positive expression rate of NY-ESO-1 protein was significantly different from the pathological grade of esophageal squamous cell carcinoma and distal end(supraclavicular) lymph node metastasis(P<0.01), and had no correlation with tumor size, location and lymph node metastasis(P>0.05).