| ObjectiveDue to the limitation of traditional detection methods, there was fewer reports of the levels of hepatitis B surface antigen (HbsAg), hepatitis B e-antigen(HbeAg), alanine aminotransferase(ALT), y-glutamyltransferase(GGT) and the five-maker of hepatitis B(HBsAg, HBsAb, HBeAg, HBeAb, HBcAb)in patients with low HBV load. The main objective of this study is to detect the HBV load precisely in patients with chronic hepatitis B, and analysis the serum markers, discuss the characteristics of the serum markers in low HBV load, providing the basic data for the treatment of patients with low HBV load.Methods112 serum samples with low concentration of HBV-DNA (20<DNA<100 copies/ml),47 serum samples with high concentration of HBV-DNA (DNA>1×l07copies/ml) from out-patients and inpatients from July,2014 to February, 2015 with chronic hepatitis B were collected randomly. The levels of HBsAg and HBeAg were determined using Chemiluminesent Microparticle ImmunoAssay (CMIA). HBV-DNA concentrations were determined using ultra-sensitive real-time fluorescent PCR. The levels of ALT and GGT were determined using Automatic Chemical Analysis (ACA). Firstly, the basic characteristics of patients with chronic hepatitis B were analyzed. Secondly, the concentrations of HBsAg and HBeAg, ALT and GGT, the abnormal rate of ALT and GGT were compared between samples with low and high concentration of HBV-DNA. Then, the correlation between concentrations of HBsAg and HBV-DNA, HBeAg and HBV-DNA, HBsAg and HBeAg, ALT and HBV-DNA, GGT and HBV-DNA were analyzed. At last, concentration distribution of HBeAg was calculated, and the characteristics of the five-maker of hepatitis B were analyzed. Data were statistically processed using SPSS 19.0 and quartiles. The median of two sample groups was compared using Mann-Whitney test Two sample concentration anomaly rate is compared with the chi square test using four grid. The median of more sample groups was compared using Kruskal-Wallis test. Statistical significance (or a statistically significant result) is attained when a p-value is less than 0.05.Results1.Among the patients with low HBV load,83% cases were man,55.4% patients were 21-40 years old.2. There were significant difference between the concentration of HBsAg (Z=6.777, P<0.001) or HBeAg (Z=6.549, P<0.001) in patients with low HBV load and patients with high HBV load.3.There were significant difference between the concentration of ALT (Z=7.180,P<0.001) or GGT (Z=3.703,P<0.001) in patients with low HBV load and patients with high HBV load;There were significant difference between the abnormal rate of ALT (x2=44.08,P<0.001) or GGT(x2 1.18,P<0.05) in patients with low HBV load and patients with high HBV load.4.When the copy number of HBV was low, there was positive correlation between the concentration of HBsAg and HBeAg (P<0.001,r,=0.457), but there was no correlation between the concentration of HBsAg or HBeAg with the concentration of HBV DNA (P>0.05).5.When the HBV load was low, there was no correlation between the concentration of ALT or GGT with the concentration of HBV DNA (P>0.05).6.When the HBV load was low, group 0? accounted for the largest percentage,64.28%. Group 0? accounted for the lowest percentage,2.68%. There were no significant difference between the concentrations of HBeAg in groups with different concentration of HBV-DNA.7.When the copy number of HBV-DNA was low, the largest group is HBsAg, HBeAb and HBcAb positive. The next group was HBsAg, HBeAg and HBcAb positive. The median of concentration of HBV DNA in group with HBsAg, HBsAb, HBeAg and HBcAb positive was largest. There were no significant difference between the concentrations of HBV-DNA in these groups.ConclusionsBy using the method that can detect HBV DNA more sensitively, we can know more about the virus replication in patients with low HBV load. When the HBV load is low, the level of HBV replication is low also. The levels of HbsAg, HbeAg, ALT or GGT are not related with the copy number of DNA, exhibit their own serum characters. |
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