Analysis Of Organic Acids In Five Species Rosaceae Fruit Herbs

Objective:1.Consider total organic acids, unsaturated fatty acids, small molecules of water soluble organic acid and triterpenoid acid extraction, separation and determination of content in five kinds of Rosaceaes fruit and lay a foundation for the research of Fruit Herbs of organic acid.2.Establish five kinds of Rosaceaes fruit to require more organic acid content index measurement system and provide a experiment method for the evaluation of medicinal materials of organic acid.3.Establish separation of different polarity organic acids with chromatographic to test five Rosaceaes fruit herbs, which can provide different polarity of organic acid test methods.4.Discuss different varieties Rosaceaes fruit of medicinas of organic acid composition and content differences and provide medicinal materials research experimental data in five kinds of Rosaceaes.Methods:1.Due to the great differences in the medicine organic acids composition and the strength, the direct titration are difficult to obtain ideal titration jump range, the potential return titration Rosaceae five gets the total organic acids content in fruit.2.To establish a method of GC-MS for analyzes organic in five kinds of Rosaceaes fruit.3.Using High performance liquid chromatography(HPLC) method,based on five kinds of Rosaceae fruit gets water soluble organic acid content determination.4.Using high performance liquid chromatography(HPLC) method for five kinds of Rosaceaes fruit herbs triterpenoid acid content determination.Results:1.Established the qualitative and quantitative methods of organic acids in five kind Rosaceaes fruit and five kinds of Rosaceae fruit kind of medicinal materials of different kinds of organic acid content determination.2.Using potentiometric back titration of water-soluble total organic acid content determination in five Rosaceae fruits herbs.The results show that the five Rosaceae fruit different origin to gets the differences of total organic acids content, including content discretion of Ebony fruits>Hawthorn fruit>Chaenomelisfruit> Rosae laevigatae Rubis fruit, which average content is 16.20%, 8.38%, 7.43%, 3.97%, 3.58%.The total organic acids content in different regions of the medicinals of the highest is fully21.95% of Fujian Ebony fruits, 8.84% He Nan Hawthorn fruits, 7.43% of Xuan Cheng Chaenomelis fruit,3.97% of Jiang Xi Fructus Rosae laevigatae1 and 3.58% of Zhe Jiang Rubis fruit.3. The method of GC-MS Rosaceae fruit kind of medicinal materials of organic acid composition analysis results :(1) the five kinds of medicinal powder after directly determined treatment, it has been determined by GC-MS with as many as 20 organic acids, organic acids have malic acid, fumaric acid, succinic acid, citric acid, 4-ketone pentanoic acid, hexadecanoic acid, oleic acid, linoleic acid, stearic acid, linolenic acid,arachidic acid, etc.With ketone of malic acid, citric acid, 4-pentanoic acid, oleic acid,linoleic acid content is relatively high in the five kinds of medicinal materials.Determined directly by the above results, the main organic acids in citric acid,malic acid, oleic acid, linoleic acid, five kinds of medicinal materials of the highest components of organic acids in Xuan papaya oleic acid 34.88%.Respectly, He Nan hawthorn, Fu Jian ebony citric acid 55.21%, linoleic acid 18.07% ketone of Jiangxi Fructus Rosae laevigatae1 4-pentanoic acid, Zhe Jiang 1 Raspberry linoleic acid 15.63%and 33.27%.(2) After extracting methyl: By comparing the 50% ethanol extraction and n-butanol extraction organic acid methyl ester is changed after the test results, five kinds of medicinal herbs, papaya malic acid content in the highest at 61.44%, He Nan hawthorn,central Asia oleic acid content is higher, at 23.45%, and citric acid in plum, Raspberry supreme respectively 59.63%, 27.98%, Fructus Rosae laevigatae from central Asia oleic acid was 17.54%.After the ethyl acetate extract and 90% ethanol extraction organicspecies are similar, but the relative content change.Detect organic acids in unsaturated fatty acid, oleic acid and linoleic acid, one of the highest relative content xuan papaya in oleic acid, respectively, as high as 51.85%, He Nan hawthorn linoleic acid is 35.29%,Fu Jian ebony linoleic acid is 18.07, Jiang Xi Fructus Rosae laevigatae1 oleic acid from central as high as 32.22%, raspberry central Asia oleic acid relative content as high as42.73%.Found by comparing the organic acid content of the differences between different medicinal materials for the organic acid types and relative content of difference between different medicinal materials.4.Set up five kind of Rosaceae fruit gets seven kind of HPLC method for the determination of water soluble organic acid content, using gradient and multiple wave length switching method to simultaneously determine the quinic acid, malic acid and shikimic acid, citric acid, fumaric acid, protocatechuic acid, the content of chlorogenic acid.Five kinds of medicinal herbs, average water soluble organic acid content of high and low order roughly Chaenomelisfruit >Rosae laevigatae>Ebony fruits>Hawthorn fruit>Rubis fruit.The average content of 2.31%, 1.60%, 0.738%, 0.63%, 0.738%, five kinds of medicinal herbs of the communist party of China some organic acids have malic acid, shikimic acid, protocatechuic acid.Papaya among the highest in water soluble organic acids in Xuan papaya shikimic acid, the content is 1.15%, ebony medicinal materials of organic acids in Fu Jian ebony citrate on highest, at 1.55%, in the Shan Dong 2 Hawthorn organs in the citrate as high as 0.42%, Fructus Rosae laevigatae medicinal materials in Jiang Xi Fructus Rosae laevigatae 1 quinic acid content in the highest is 0.30%, and Raspberries of Zhe Jiang1 folic acid content is the highest of0.52%.5. Based on the five kinds of triterpenoid acid, LOQ determination found that five kinds of triterpenoid acid are low, hawthorn acid, birch, barroso acid ester acid, oleanolic acid and ursolic acid detection limit were 4.39 ng/m L, 6.38 ng/m L, 10.39 ng/m L, 10.14ng/m L, 10.78 ng/m L, LOD were 1.36 ng/m L, 2.16 ng/m L, 3.21 ng/m L, 3.18 ng/m L,3.19 ng/m L.Experiment results found in the material liquid with high than addinganhydrous ethanol ultrasonic 60 min extract is better.By the test results found that five kinds of medicinal materials of papaya and hawthorn can detect five triterpenoid acid,plum herbs can detect the four kinds of triterpenoid acid can be detected in Fructus Rosae laevigatae and Raspberry and three.Five kinds of medicinal herbs in average content of triterpenoid acid in high and low order Rubis fruit>Chaenomelisfruit>Ebony fruits>Hawthorn fruit>Rosae laevigatae, content is respectively 0.357%, 0.27%, 0.357%,0.153% and 0.076%.Five kinds of triterpenoid acid ursolic acid content is higher, in different regions of medicinal materials in Zhe Jiang 1 Raspberry ursolic acid content as high as 0.40%.Fructus Rosae laevigatae of Jiang Xi2, unknown origin of ebony ursolic acid content in the lowest 0.052%.Conclusion:This topic through the potential return titration, GC-MS, HPLC-UV single wavelength and wavelength switching of five kinds of Rosaceaes fruit gets organic acids are studied.Potential method for the five kinds of medicinal herbs flavour the size of the values of "acid";GC-MS illustrates the five kinds of medicinal herbs in the main types and relative content of organic acid.HPLC determination of five kinds of medicinal materials of water-soluble organic acids and triterpenoid acid, found that contain water soluble organic acids on the species and the total content were higher than triterpenoid acid.This topic provides a method and the experimental data for a variety of medicinal components of organic acids system research.