The Research For The Treatment Of C6 Glioma Rat Models Treated By Newcastle Disease Virus FMW Strains

Objective:This study aims to investigate wheather Newcastle Disease Virus(NDV)FMW strains have the ability of suppressing growth of C6 cell glioma,and providing a theoretic basis for clinical treatment.Methods: 100 SD rat models has been established by stereotactic apparatus after cluturing,collecting and concetrating the C6 cells.Each of the rats model had been inoculated the cells into the right basal ganglia.7 days later,the models were examined by MRI and calculated the volume of them.Discarding the failed models and screening out 36 models which tumor volume and survival condition are similar,then divided all the 36 models into 3 groups,every group 12 rats.Group A: group A is a nagtive control group and the rats model in this group inoculate C6 cells only.Group B:rats in this group were injected 107TCID50 NDV through the tail vein.Group C:rats in this group were received intratumoral injection with 107TCID50 NDV by stereotactic apparatus.Record and compare the suvival time of the rats of each group.Make and observe tumour biopsies,and TUNEL assy and FCM were used to detect apoptosis.Results:(1).The C6 glioma rat modles has been established and screened.(2).The survival time of rat models of two treatment group are longer than control group,and in these three groups,suvival time of the rats in the group which were received intratumoral injection with 107TCID50 NDV by stereotactic apparatus is the longest.TUNEL assy and FCM’s result show that NDV FMW can induce the C6 glioma cells apoptosis.Conclusion:(1).TUNEL assy and FCM were used to detect apoptosis,and the results revealed NDV could induce the C6 cell apoptosis.(2).TUNEL assy results revealed that the number of apoptotic cell of the rats which used local treatment are more than systemic treatment group,and the survival time of local treatment group is longer than systemic treatment group.These results indicate that the therapeutic effect of injection NDV in situ by stereotactic apparatus is better than systemic treatment by injecting NDV through the tail vein.