Quality Standards And Establishment Of Fingerprint Of Safflower

With the continuous research and development of pharmacy, the efficacy of Chinese medicine has gradually in clinic has been a large number of applications, with the market value and the economic value increasing, the quality of Chinese medicine is uneven, some good and some bad, species complex sources and processing technology of chaotic phenomenon. Safflower is safflower injection preparation of raw materials, clinical medicine quality will directly influence the injection. With the supervision department of clinical drug administration efforts to increase, pharmaceutical companies must the medicinal quality control, the establishment of quality standard system, medicinal herbs to regulate the origin, picking in assurance of safflower injection quality in clinical application.Through of Flos Carthami moisture, total ash, absorbance, extract and hydroxy safflor yellow A, the content of kaempferol were determination of Flos Carthami to study the quality standard. Application of high performance liquid chromatography (HPLC) to establish the fingerprints of safflower.In the batch quality standard of Flos Carthami, different areas, and in water, total ash, determination of absorbance and extract largely in line with the corresponding testing standard, the moisture content of about 8%-12%, the total ash content in 9%-14%, absorbance at 0.3-0.5, extract at 35%-48%, the content of hydroxysafflor yellow A at about 1.36%-1.71%, the content of kaempferol is about 0.6%-0.8%, we examine each test item of Flos Carthami from different aspects, found safflower of Xinjiang safflower from the overall level was higher than other areas, picking date in mid July to early August is the best harvest date, processing and storage of environmental medicine is the factory must be clearly stated in the purchase of Flos carthami.In the process of establishing the fingerprint of Flos Carthami, determine the optimum chromatographic conditions, using YMC C18 (250mm* 4.6,5 m) column, acetonitrile -0.05% three trifluoroacetic acid as the mobile phase. The detection wavelength was 223nm. The column temperature was 30 C, flow rate lml/min, the injection volume was 10 L, with the peak of A hydroxy safflower yellow is the characteristic peak of 10 common peaks were confirmed, to establish the fingerprint of Flos Carthami, consistent with the overall appearance of the main peaks of fingerprints obtained from different medicinal group and the distribution, but in different areas of Xinjiang, the fingerprint of Flos Carthami is high, high content and characteristic peaks of hydroxysafflor yellow A, in the purchase of Chinese herbal medicine Xinjiang safflower is a good choice, relatively stable in a specific area of medicinal components, physical and chemical properties is not easy to change.