| Objective: This study aimed to detect the expression level of Shank1 in hepatocellular carcinoma(HCC),and investigate the difference of them between the tumor and adjacent tissue,and correlate them with the clinical pathological characteristics of HCC.Methods: The carcinoma tissue and carcinoma adjacent tissue of 75 HCC patients were selected.The expression level of Shank1 was detected by immunohistochemistry and western blot.Statistical analysis was performed to determine the relationship between the expression of Shank1 and the clinicopathological features,including sex,age,tumor size,lymph node metastasis and clinical stage of HCC patients.Results: Results of immunohistochemistry showed that the expression level of Shank1 in liver cancer tissue was significantly lower than that in the carcinoma adjacent tissue.Down-regulation of Shank1 was 73.33%(55/75),and the difference was statistically significant(P<0.001).Western Blot resultes showed that the expression level of Shank1 in cancer tissue was also significantly lower than that in the carcinoma adjacent tissue.Correlation analysis found that the low expression level of Shank1 in liver cancer tissue was not significantly related to gender,age,tumor size and lymphatic metastasis,but was significantly associated with the clinical stages of HCC(P<0.05).Conclusion:1 The expression level of Shank1 protein in HCC was significantly lower than that in the adjacent tissues,suggesting that the expression level of Shank1 was down-regulated in HCC.2 the expression level of Shank1 protein was significantly correlated with the clinical stage of HCC,suggesting that the down-regulated expression of Shank1 may be related to the malignant behavior of HCC.3 Therefore: Shank1 is abnormal expression in HCC cancer tissues,and can be used as the potential objective indicators of diagnosis and clinical staging,also has further research value. |
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