Effect Of Casein Glycomacropeptide In Mice With Ulcerative Colitis Intervened By Intestinal Cell Apoptosis And Realted MRNA Transcription

In order to explore the effect of casein glycomacropeptide(CGMP)on the apoptosis and apoptosis pathway of Oxazolone-induced Ulcerative Colitis,It is very valuable to study the intestinal epithelium apoptosis and the formation of IBD,the relationship of the pathogenesis and treatment.After the success of modeling,three treatment groups were administered CGMP at three doses of 5 mg /(kg bw·d),50 mg /(kg bw·d)and 500 mg /(kg bw·d)and drug was administered sulfasalazine orally 40 mg /(kg bw·d)by oral gavage on xazolone-induced ulcerative colitis in mice,In addition,control group and model group were administered a daily dose of ultra-pure water.Using HE staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay(TUNEL assay)to detect gross morphology,histological damage and colonic epithelial cells apoptosis,Using RT-PCR to detect the relative experssion of FasL、TNF-α、p53、p65、TGF-β1 mRNA in Oxazolone-induced Ulcerative Colitis(UC)mice.Treatments with the three doses of CGMP showed improvement on oxazolone-induced ulcerative colitis,while the dose of 50 mg /(kg bw·d)、500 mg /(kg bw·d)CGMP seemed to be better which was generally comparable to that of sulfasalazine,and treatments with the three doses of CGMP showed inhibition of FasL,p65 mRNA experssion,In particular,the most obvious role of middle and high doses.It played a signicant role through the FasR / FasL pathway to inhibit intestinal epithelial cell apoptosis and through the NF-κB p65 pathway to prevent the occurrence of inflammation.Low and middle doses of CGMP promote the expression of TGF-β1 mRNA,but high dose and drug play side-effects to inhibit its expression.It is no signifcant change of the expression of TNF-α and p53.In conclusion,CGMP,a biological active substance,holds promising potential to be applied in a nutritional therapy for the treatment of ulcerative colitis by FasR/FasL to inhibit the apoptosis and NF-κB p65、TGF-β1 pathway to inhibit the inflamation.