| objective: By observing the effect of Tongqiaohuoxue Decoction on neurological function of rats, NSE, BDNF, SOD, MDA and brain edema in acute cerebral hemorrhage,To investigate the preventive effect and mechanism of Tongqiaohuoxue Decoction cerebral hemorrhage,To provide a reliable experimental basis for the treatment of cerebral hemorrhage with the method of promoting blood circulation to remove blood stasis.Methods : 1. Grouping: Taking eighty four male SD rats weight in(250 ± 20)g were randomly divided into sham operation group, model group, edaravone group(Western medicine group), Tongqiao Huoxue Decoction high dose group(high dose group), Tongqiao Huoxue Decoction middle dose group(referred to as "agent group"), Tongqiao Huoxue Decoction low dose group(low dose group), 14 rats in each group;2 modeling methods: Using the methods of rat tail autologous blood injection molding method;3. dministration: The western medicine group were given edaravone 1.5mg/kg/d intraperitoneal injection,High, medium and low dose group were treated with Tongqiaohuoxue Decoction 1.2g/Kg/d, 0.6g/Kg/d, 0.3g/Kg/d gavage,The sham operation group and the model group were given the same volume of distilled wate by intragastric administration.After the model was completed, Longa Zea 5 method was used to evaluate the neurological deficit scores in each group,selecting neural function defect score in 1-3 points as to the drug delivery object; Then, the sham operation group and model group were given the volume of normal saline to fill the stomach.Low, middle and high dose group of Chinese medicine and Western medicine group, such as the above method of administration to 1 weeks;4 sampling:1 weeks later, each group were abdominal aortic blood sampling;after blood sampling, the hearts were perfused and the brain tissues were taken Immediately.5 indexes: To detect SOD activity by xanthine oxidase method,and The content of MDA was detected by the method of acid(TBA);ELISA method for the detection of NSE content;BDNF content was detected by immunohistochemistry,Observation of the morphological changes of nerve cellsaround hematoma after intracerebral hemorrhage by light microscope.Results:(1)Comparison of neurological deficits score:Compared with the sham operation group, the scores of neurological deficits In the rest groups were increased,, the difference was statistically significant(P < 0.05).Compared with the model group, the score of neurological deficits In low dose group was decreased, but the difference was not statistically significant(P > 0.05), the neurological deficits score In the middle and high dose group and Western medicine group was significantly lower,the differences were statistically significant(P < 0.05).Compared with the low dose group, the neurological deficits score In the middle and high dose group and Western medicine group was lower,the difference was statistically significant(P < 0.05).Compared with the middle dose group, the neurological deficits score in High dose group and western medicine groupdecreased, the difference was statistically significant(P < 0.05).compared with the western medicine group,the neurological deficits score changes of the High dose group was not obvious,there was no significant difference between them(P > 0.05).(2)Comparison of SOD activity:Compared with the sham operation group, the SOD activity in The rest of each group decreased, the difference was statistically significant(P < 0.05).Compared with model group, Except for the sham operation group, the SOD activity increased in the other groups,the difference was statistically significant(P < 0.05).Compared with the low dose group, The activity of SOD in middle and high dose group and Western medicine group was increased,and the difference was statistically significant(P < 0.05).Compared with the medium dose group, The activity of SOD in high dose and Western medicine group was increased, the difference was statistically significant(P < 0.05).Compared with western medicine group, the activity of SOD was increased in high dose group,and the difference was statistically significant(P < 0.05).(3)Comparison of MDA content: Compared with the sham operation group, the MDA content in The rest of each group increased,the difference is statistically significant(P < 0.05).Compared with model group, the content of MDA in low dose group decreased,the difference was not statistically significant(P > 0.05), The content of MDA in middle and high dose group and Western medicine group was significantly decreased, the difference is statistically significant(P < 0.05). Compared with the low dose group, the content of MDA in middle and high dose group and Western medicine group was significantly decreased,The difference was statistically significant(P < 0.05).Compared with the middle dose group, the content of MDA in high-dose group and Western medicine group was decreased, and the difference was statistically significant(P < 0.05).Compared with western medicine group, the content of MDA was decreased, but the difference was not statistically significant(P > 0.05).(4)Comparison of the content of BDNF:Compared with sham operation group, the content of BDNF in other groups increased, the difference was statistically significant(P < 0.05). Compared with model group, the expression of BDNF in low dose group increased, but the difference was not statistically significant(P > 0.05). The expression of BDNF in middle and high dose group and Western medicine group was significantly increased, and the difference was statistically significant(P < 0.05). Compared with the low dose group, the expression of BDNF in middle dose group increased, but the difference was not statistically significant(P > 0.05). The expression of BDNF was significantly increased in high dose group and Western medicine group, and the difference was statistically significant(P < 0.05). Compared with the medium dose group, the expression of BDNF in high dose group and Western medicine group was significantly increased, and the difference was statistically significant(P < 0.05). Compared with western medicine group, the change of BDNF was not obvious, and the difference was not statistically significant(P > 0.05).(5)Comparison of the content of NSE:Compared with the sham operation group, NSE levels in Model group, low and middle dose group were significantly increased,the difference is statistically significant(P < 0.05); The content of NSE in high dose group and Western medicine group was not obvious,the difference was not statistically significant(P > 0.05). Compared with the model group, the content of NSE in the low dose group was not significant, the difference was not statistically significant(P > 0.05), The content of NSE in middle and high dose group and Western medicine group was decreased, the difference was statistically significant(P < 0.05). Compared with the low dose group, The content of NSE in middle and high dose group and Western medicine group was decreased,and the difference was statistically significant(P < 0.05). Compared with the middle dose group, the content of NSE in high dose group and Western medicine group was lower, and the difference was statistically significant(P < 0.05). Between High dose group and Western medicine group, NSE content change is not obvious, the difference was not statistically significant(P > 0.05).(6) Comparison of brain tissue water content:Compared with the sham operation group, Water content of brain tissue in the other groups increased, the difference was statistically significant(P < 0.05). Compared with the model group,Water content of brain tissue in the low dose group, middle dose group, high dose group and Western medicine group was reduced, the difference was statistically significant(P < 0.05). Compared with the low dose group, Water content of brain tissue of the middle dose group, high dose group and Western medicine group was decreased, and the difference was statistically significant(P < 0.05). Compared with the middle dose group, there was a significant difference between the high dose group and Western medicine group(P < 0.05). Compared with western medicine group, the change of water content of brain tissue was not significant, and the difference was not statistically significant(P > 0.05).(7)The results of light microscope: The morphology of nerve cells around the hematoma was observed under light microscope. The structure of the peripheral nerve cells in the sham operation group was intact, and the cytoplasm and nucleus were evenly stained, and no abnormal changes were found. In the model group, there were edema, tissue structure loose, nerve cell necrosis and a small amount of inflammatory cell infiltration around the hematoma. Tongqiao Huoxue Decoction low, medium and high dose group showed different degrees of brain tissue edema and inflammatory cell infiltration, but with the dose increased, edema and inflammatory reaction decreased gradually. In western medicine group, the structure of peripheral nerve cells was relatively complete, and there was no obvious brain edema and inflammatory cell infiltration.Conclusion:1 Tongqiaohuoxue decoction can improve the neurological unction of rats with cerebral hemorrhage deletion,To alleviate the egeneration and necrosis of nerve cells and inflammatory cell infiltration in the peripheral nerve after intracerebral hemorrhage.2 Tongqiaohuoxue decoction has a certain dose-response relationship in the prevention and treatment effects of intracerebral hemorrhage in rats on.3 possible mechanisms:1.Tongqiaohuoxue decoction can Improve blood circulation in patients with cerebral hemorrhage before and after cerebral hemorrhage,This may be related to Rhizoma Chuanxiong, radix paeoniae rubra, safflower, peach kernel which can promot blood circulation and removing blood stasis Relevant role.2.Tongqiaohuoxue decoction can reduce the content of MDA, increase the activity of SOD to scavenge oxygen free radicals, thereby protecting nerve cells.3.Tongqiao Huoxue Decoction can promote the absorption of hematoma, reduce inflammation and brain edema and reduce the nerve cell injury induced by ischemia and hypoxia.4.Tongqiaohuoxue decoction can promote cerebral hemorrhage after neurotrophic factor expression, protect nerve cells. |
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