Association Between Single Nucleotide Polymorphisms(SNPs) Of Filaggrin(FLG) Gene And The Atopic Dermatitis(AD) In Yanbian Chinese Koreans

Objective:To investigate the association between single nucleotide polymorphisms(SNPs)of the gene of filaggrin(FLG)and atopic dermatitis(AD)in Yanbian Korean Chinese populations.Methods:The project was designed as a case-control study.A total of 70 subjects withAD,and 90 control subjects with were involved in this study.The genotypes and alleles of SNPs rs11584340and rs3126085 were determined by the method of Polymerase Chain Reaction Sequence Method.The distribution of allele and genotype frequencies were determined to screen dangerous haplotype and physiological and biochemical indexes among the genotypes were compared in AD and the normal group.Results:①The sample balance test:AD and the normal group,the genotype and allele frequencies of 2SNP rs11584340(G),rs3126085(G)conformed to Hardy-Weinberg equilibrium and showed no significant difference(p>0.05);②Analysis of the genotype and allele frequencies of FLG gene 2SNP distribution between AD and controls case:In Yanbian Korean Chinese population,both allele and genotype frequency of rs11584340 and rs3126085 showed no significant difference between patiens with AD and healthy control group(p>0.05);③ Genotype of 2SNPs and clinical biochemistry indicator:Genotype AG of rs11584340 showed high serum IgE(P=0.050),but no significant differences with IL-13(p=0.661);④Clinical biochemistry indicator:In Yanbian Korean Chinese population,the level of serum IgE and IL-13 in AD was significantly higher than in control group(P<0.01);⑤Analysis of linkage disequilibrium:rs11584340 was strong linkage disequilibrium with rs3126085(D’=1.000,r2=0.836);⑥Nation,race comparion:The alleles frequencies of 2 SNPs in yanbian Korean Chinese populations had significant differences with that in HapMap database(ss41111533,ss17361789).The allele G frequency of rs11584340 in yanbian Korean Chinese was significantly lower than Yoruban in Ibadan(YRI)and European(CEU)(p<0.01)and higher than Japanese in Tokyo(JPT)(p<0.05),but no significant differences with Han Chinese in Beijing(HCB)and Japanese in Tokyo(JPT)(p>0.05).The frequencies of the alleles G of rs3126085 was significantly lower than CEU(p<0.01),but had no differences with HCB、JPTand YRI(p>0.05).Conclusions:①Genotype AG of rs11584340 showed high serum IgE of AD in Yanbian Korean Chinese population;②The serum IgE and IL-13 is associated with susceptibility of AD in Yanbian Korean Chinese population,is the risk factor for AD;③rs11584340 was strong linkage disequilibrium with rs3126085;④FLG gene SNPs had nation difference and race difference;⑤The polymorphism of rs11584340 and rs3126085 is not associated with susceptibility of AD in Yanbian Korean Chinese population.