The Protection Of Mangiferin On Myocardial Injure In Diabetic Rats

ObjectiveWe observed Mangiferin’s effects on myocardial injury in diabetic rats,and explored its possible mechanism in the process.They provide a newbasis for clinical prevention and treatment on diabetic cardiomyopathy.Methods40healthy rats, randomly selected from50male healthy rats, were injectedSTZ45mg/kg in the tail veins. Another10injected the same amount of citratebuffer as a control group.72h, Fasting blood glucose was measured withSureStep Plus detector ainfter72h, blood glucose of the diabetes model was≥16.7mmol/L.The model rats were randomly divided into diabetic model group(DM), Mangiferin low, medium and high dose groups (15mg/kg/d,30mg/kg/d,60mg/kg/d).Each group had nine rats. The treatment groups of Mangiferin weregiven Mangiferin at different does for12weeks, and the remaining groups werereceived equal volumes of saline.After12weeks, fasting blood glucose wasmonitored by SureStep Plus;HWI and LVMI of Rats were measured byBalances; Cardiac function was observed in rats with biomedical image analysissystem;HE, Masson staining were used to detect myocardial histopathologychanges; expressions of AP-1, TGF-β1and FN were detected byimmunohistochemistry and Western Blot.Results1.Compared with the normal group, Blood sugar of the model group wassignificantly higher (P <0.01), HWI and LVMI were increasedsignificantly (P<0.01), LVEDP was significantly rised (P <0.01), LVSP,±dp/dtmax wereremarkably dropped (P <0.01); compared with the model group, In mangiferindose groups blood sugar was down(P <0.01), HWI and LVMI were decreased(P <0.01), LVEDP lower (P <0.01), LVSP,±dp/dtmax were increased (P <0.01), and these changes were most significant in the high dose group ofmangiferin (P <0.01).2. The morphologically of myocardial cells was regulared and muscle fiberswere arranged regularly in the normal group; In the model group myocardial cellvolume significantly increased,myocardial fibers were disorganized andcollagen fibers elevated remarkably; compared with the model group, Thepathological changes were improved in the does groups of Mangiferin. In thehigh dose group of mangiferin myocardial tissues were returned to normal andcollagen fibers. decreased significantly.3. Compared with the normal group, The expressions of AP-1, TGF-β1, FNwere notably rised in the model group(P <0.01); compared with the model group,In dose groups of Mangiferin the expressions of AP-1, TGF-β1, FN expressionwere reduced (P <0.01), and by the dose-dependent manner (P <0.01).Conclusion1. Mangiferin can reduce blood glucose in diabetic rats.2. Mangiferin has a protective effect to diabetic myocardium, can reducemyocardial injury, improve cardiac function, inhibit proliferation of collagen fibersand prevent myocardial fibrosis.3. Mangiferin against diabetic cardiomyopathy may be related to inhibition thexpression of AP-1, and thus reduced TGF-β1, FN.