Variation Analysis Of Wheat Storage Protein And Genome

Wheat is one of the world’s four major food crops,about 35% of the global population as staple food.Due to China’s long-term directional selection breeding of wheat,narrowing the genetic basis of cultivation of wheat,for new breeding methods and the need of new genetic material is very urgent.The ethyl methyl sulfone(EMS)mutation has become a commonly used method of breeding in wheat breeding.In addition mutation breeding technology application in crops and distant grafting is an innovation in the field of plant breeding,it can take advantage of anvil head wide source and anvil grafting technology,the advantages of greatly expand the genetic basis.But now the genetic mechanism of grafting induced cause is still unclear,such as the application of induced grafting technology in breeding has certain obstacles.Therefore,the study of the relationship between grafted and grafted progeny,reveal the molecular mechanism of genetic variation and formation mechanism of grafting,grafting for the rational use of induced mutation technique in plant breeding and the theoretical basis of reference.This study through the detection of mutation in DNA fragment by SSR marker differences between types and their parental control,and the variation of A-PAGE and SDS-PAGE proteins by gel electrophoresis was used to detect the mutant type of gliadin and HMW glutenin subunits,provide evidence for application of EMS mutation and distant grafting breeding at the protein level and molecular level.Mutation and distant mutation technology on wheat.And to observe and analyze the quality difference between two kinds of mutant offspring breeding and genetic diversity.The results are as follows:1.The material between groups of different wheat varieties EMS mutagenesis and grafting exist among groups of mutagenic material both relatively extensive gliadin genetic variation.Zhoumai 22 EMS mutation group 41 accessions with parental gliadin appeared variation,there were 38 gliadin patterns,parents and progeny genetic distance was between 0.0213 ~ 0.4762,the similarity coefficient was 0.51 clustered into 2 branches;Zhoumai 27 EMS population by 29 and the parent material between gliadin variation appeared,there were 27 gliadin patterns,parents and progeny genetic distance was between 0.0476 ~ 0.3143,the similarity coefficient was 0.65 clustered into 2 branches;Bainong 207 EMS population by 16 materials with parental alcohol soluble protein appeared variation,there were 29 gliadin patterns,parents and progeny genetic distance was between 0.0303 ~ 0.5238,the similarity coefficient was 0.33 clustered into 3 branches;Zhengmai 7698 EMS population by 11 and the parent material between gliadin variation appeared,there were 29 gliadin patterns,parents and progeny genetic distance was between 0.0769 ~ 0.5333,the similarity coefficient was 0.59 clustered into 3 branches.Grafting induced 6 groups 23 material between the parent and a variation in the gliadin zone type are not the same each other,a total separation of 38 kinds of gliadin zone type.The genetic distance between parents and offspring material are: Wenmai 19 and Wenmai 19 grafting mutagenesis progeny(0.4545);Yumai 14 and Changyi 14(0.5556);Aikang 58 and Changyi 9(0.3333);Zhoumai 18 and Zhoumai 18 grafting mutagenesis(0.0769);Yumai 18 and Yumai 18 grafting induced offspring(0.1538 ~ 0.5758),with a mean of 0.2889.Yumai 34 and Yumai 34 grafting mutagenesis(0.5000).Its group material clustering results found that Zhoumai 18 of wheat gliadin differences between it and its grafting mutagenesis progeny smaller;Yumai 18 grafting mutagenesis progeny Changyi 5 with Yumai 18-5 mutagenic six gliadin genetic variation between different segregation was not significant,but its parent Yumai 18 gliadin variation is relatively obvious.The rest of the grafting induced gliadin genetic variation between groups materials have high polymorphism.2.Through the EMS induced mutagenesis and grafting between groups and their parents HMW-GS analysis showed that two kinds of different mutagenesis method to produce a large amount of HMW-GS types of variation.Zhoumai 22 EMS mutagenesis progeny 41 wheat materials there’re a total of 24 in relative to the parent 1,7 + 9,5 + 12 combination type change,its subunits variation types and mutation frequency was: Null(36.59%),7 + 8(19.51%),17 + 18(2.44%),2 + 12(34.15%),5 + 10(4.88%).Zhoumai 27 EMS mutagenesis there’re a total of 9 generations of 29 materials relative to the parent 1,7 + 8,5 + 12 combination type variable,its subunits variation types and mutation frequency was: Null(13.79%),7 + 9(10.34%),5 + 10(17.24%),2 + 2 + 12(10.34%),10(3.45%).Bainong 207 offspring induced by EMS 16 A total of 7 accessions of relative to the parent 1,7 + 9,5 + 12 combination type change,its subunits variation types and the mutation frequency is: Null(37.50%),7 + 8(6.25%),2 + 12(37.50%).Zhengmai 7698 EMS mutagenesis progeny there’re a total of 9 november material relative to the parent 1,7 + 9,5 + 10 combination type change,its subunits variation types and mutation frequency was: Null(18.19%),7 + 8(54.55%),5 + 12(63.64%),2 + 12(35.29%).Wenmai 19,Yumai 14 and Zhoumai 18 three grafting materials between offspring and parent without HMW-GS mutation,the other groups are varied in different subunit types.The subunit composition of Changyi 9 grafted Mutation Progeny materials from 1,7+8,5+10,7+9,2/5+12,variation of Null subunit composition.Yumai 18-64(Null,14+15,5+12)subunit mutation type and mutation frequency of 7+8(8.33%),7+9(91.67%),2+12(100%).Subunit composition of Yumai 34 grafted Mutation Progeny materials from 1,7+8,5+10,7+9,2+12,variation of Null subunit composition.3.After the EMS Genotype Wheat Mutation group and mutation group grafted materials materials were PCR amplified polymorphism analysis:EMS mutagenesis,57 of the polymorphic,detected 519 allelic variation.Each of 2.00 ~ 20.00 primers can amplify the allelic variation,each pair of primers average allelic variation of 9.11.And its polymorphism loci of Nei’s genetic diversity index of different loci(He)the variation range of 0.0997 ~ 0.0997,with an average of 0.7311;Genetic polymorphism information content index PIC value variation range of 0.0948 ~ 0.0948,with an average of 0.7044.Cluster analysis results showed that SSR markers can accurately reflect the Zhoumai 22,Zhoumai 27,Bainong 207 and Zhengmai 7698 four wheat EMS mutagenesis material,the genetic diversity between the four groups and EMS mutagenesis were alone clustering for each one.Grafting mutagenesis,the 69 pairs of primers,polymorphism detected a total of 1041 allelic variation.In which each pair of primers can amplify the 9.00 ~ 21.00 allelic variation,each pair of primers average allelic variation of 13.52.And its polymorphism loci of Nei’s genetic diversity index of different loci(He)the variation range of 0.4896 ~ 0.4896,with an average of 0.8426;Genetic polymorphism information content index PIC value variation range of 0.4774 ~ 0.4774,with an average of 0.8292.Clustering analysis results show that although no clustering between 10 common wheat varieties on a separate branch,but also not alone with other grafting material clustering to illustrate these grafting offspring and not from the common wheat cultivars.Wheat parents Wenmai 19,Yumai 14,58,Zhoumai 18 and Yumai 34 were grafted with offspring clustering as a group;Yumai 18 grafting offspring with Changyi 1 clustering as a group,while the rest of the grafting offspring for a single clustering.