A New Fluorescent Sequence-related Amplified Polymorphism (FSRAP) And Its Application To Mapping In Wheat

Common wheat(Triticum aestivum L.,2n=6X=42)is an allohexaploid species with A,B and D genomes.It has a large genome(about forty times of the rice genome)and a high proportion of repetitive sequence.Therefore,compare with other crops,such as corn and rice,it`s a challenges to develop molecular markers in wheat.In order to enrich the molecular markers in wheat,and lay foundation for molecular markers assisting breeding and gene mapping,a new fluorescent sequence-related amplified polymorphism(FSRAP)markers had been development in this studies base on the SRAP molecular marker.And then a genetic map of wheat was constructed base on the FSRAP makers and using the recombinant inbred lines(RILs)population derived from Chuanmai42×Chuannong16 by single seed descent as mapping population.The main results were described as following:1.In this study,a new FSRAP molecular marker had been development.The stability and sensitivity experimental results indicated that FSRAP showed high sensitivity and stability compared with SRAP markers.2.A total of 1936 primer combinations were combined using 44 fluorescently labeled forward primers and 44 reverse primers.There were 260 primer combinations in the 1936 pair of primers showed polymorphism in the parental materials,Chuanmai 42 and Chuannong 16.Further screening using 9 individual plants from the RILs population,finally,189 primer combinations with polymorphism were screened out.3.189 primer combinations produced 430 FSRAP markers in the RILs population,average 2.28 markers for each primer combination.The 113 primer combinations(about59.79%)produced two or more than two makers.Three primer combinations produced the most markers,namely,Me24-Em21,Me25-Em8 and Me32-Em10,and each primer combinations produced ten markers.4.A total of 281 FSARP markers and 39 SSR markers were placed onto 20 linkage groups(no FSRAP markers distributed on 7D chromosome).The maps spanned a total length of 2499.3c M,and the average distance was 7.81 c M.The nearest two markers were M17E32 A and M40E21,which located on the 1B chromosome,only0.3c M apart.While the farthest markers were Xcfd19-D and M8E40 A,which located on the 1D chromosome,the genetic distance reached 48.8c M.There are 201 makers(including 184 FSRAP markers and 17 SSR markers)mapped on the B genomes and covered distances of 1013 c M.On the A genomes there are 84 markers(including 71 FSRAP markers and 13 SSR markers)and covered distances of 849.6c M.On the Dgenomes,however,only 35 markers and covered distances of 636.7c M,and there were no FSRAP markers distributed on 7D chromosome.The 1B chromosome contained most of the markers and had 48 markers.The markers density on 1B chromosome also the largest,the average genetic distance between the two markers was 1.5c M.The 1D and 4D chromosome contained least the markers and had only 2 FSRAP markers and 1SSR markers.The 4D chromosome has the shortest genetic distance,only 53.7c M.While the 4B chromosome has the longest genetic length,and 36 markers covered distances of 269.5cM.