| Osmanthus fragrans ’s sexual system is a rare male allogeneic(male and hermaphroditc).In the natural population,the ratio of male to female is 1:1,and the high proportion of male maintenance mechanism and evolutionary power are worthy of further study.In this study,the photosynthetic physiological characteristics and reproductive traits of different sex plants were compared and the pollen-stigma interaction was analyzed by artificial pollination experiment.It was clear whether there was self-compatibility and type 2 heterozygosity,Function,from the nutrient accumulation,reproductive allocation,mating system and its process to proven the different angles of sweet and white and its relationship between the male and female in the growth of the plant,in order to further explore the evolution of plant system in the male heterosexual pathway more evidence.The main findings are as follows:1.The diurnal curves of different sex Osmanthus fragrans showed a double-peak curve on the diurnal changes of photosynthesis and flowering period and flowering stage.The diurnal changes of photosynthesis were positively correlated with the changes of stomatal conductance and the photosynthetic rate of the hermaphroditc strains was higher than that of the male plants,and the photosynthetic capacity and the maximum net photosynthetic rate were higher.From the inflorescence differentiation stage to the fruit enlargement period,the main parameters affecting the photosynthetic capacity,And the change of chlorophyll and different components in the leaves of Osmanthus fragrans has the same regularity,and the photosynthetic capacity and the manufacturing ability of the nutritive material are better than those of the other Male strain.2.Comparison of the male and hermaphroditc part of the adult flower traits,the number of branches,the total number of flower buds on the new branches,the distance between the flower buds and the amount of flowers in each section were compared with those of the male flowers.(444.22±2.64)was significantly higher than that of male plants(404.99±2.23).The number of pollen in male flowers was significantly higher than that in male flowers(17979.94±976.20 tablets)In the sexes(13564.82±820.11 tablets).3.The highest proportion of pollen normal germination stalks reached 84.44%,and there was no significant difference with the natural pollination status,indicating that there was no obstacle before fertilization of Osmanthus fragrans,and the pollen germination and growth status of both sexeswere better at accepting exotic pollen,the affinity of male plant pollen and the growth of pollen tube were better than that of hermaphroditc and natural pollination,and there was incompatibility in different pollination types,The seed setting rate of male plants was 45.05% ± 6.94%,which was significantly higher than that of the sexes(34.54% ± 7.03%)and 28.45% ± 6.69% of the natural pollination.Therefore,the male Function is stronger than hermaphroditc strains.4.Based on the Osmanthus fragrans’ s transcriptase group,the sweet potato was used as the DNA template,and the 18 pairs of random primers were screened by the optimized EST-SSR PCR amplification system to obtain 19 pairs of high stability and rich polymorphism EST-SSR primers,the proportion of polymorphism was 12.67%,and the primers were verified by Jiangxi Ganzhou,Fujian official and Zhejiang Qiandaohu wild osmanthus populations.Finally,the SSR primers were screened Effectiveness and applicability,which laid the foundation for the development and utilization of Osmanthus fragrans molecular markers.It also provided important basis for the construction of sweet-scented Osmanthus fragrans fingerprints,genetic diversity analysis,molecular breeding and protection,and paternal analysis. |
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