| The formation and development of fruit is the basis for the production of horticultural crops such as strawberry.The regulation effect of hormones on the fruit development and maturation is an important study field in fruits.The study on the relationship between hormones and fruit development can not only provide us a theoretical reference for the cultivation of fine varieties and improvement of fruit quality,but also provide the basis for the application of growth regulators to improve the yield and quality of fruits.In this study,we used non-pollinated forest strawberry YW5AF7 as the test material to spray young fruits with different concentrations of different hormones in early development,and at the same time to investigate the changing of FvC04 in the early development of suawberry fruit.The main aim of this study was to provide a basis for futher understanding and elucidating of the effect of exogenous hormones on the early development of strawberry fruit.The main results are as follows:1.Effects of GA3 of different concentrations on the early fruit development of YW5AF7 strawberry were clarified.All the different concentrations of GA3(400 μmol/L、500 μmol/L、600 μmol/L)showed promotion effect to the early development of strawberry fruit,with the promotion of the development of the pith and cortex tissues.The most significant effect was observed at the concentration of 500 μmol/L.2.Effects of different plant hormones(or their combination)on the early fruit development were compared and analyzed.Results showed that all the same concentration(500 μmol/L)treatments of different hormone(GA3,NAA,NAA + GA3)could promote the early development of strawberry fruit,and the effect of NAA + GA3 treatment with 500 pmol/L concentration was the best one.3.Efects of exogenous hormones on the expression of FvC04 gene in strawberry fruit was detected with the method of quantitative RT-PCR.Detection showed that compared with the control,three kinds of hormone treatment(GA3,NAA,NAA+GA3)could significantly increase the expression of this gene,which was upregulated obviously at the early fruit formation stage. |