Physicochemical Characterization And Antioxidant Activity Of Chinese Honey Of Ankang

Ankang is located in the Chinese bee breeding area, it known as the traditional apiculture, has been got a certain scale. Chinese honey of Ankang is a kind of healthy place Multi nectar. Most of the honey produced Different floral origin, the output is not high, not formed industrial scale. The color of honey in different areas from the light amber to deep amber range, and some even dark red, Crystallization degree is variant. In this paper, the physical and chemical composition of honey, selenium content, free amino acid, antioxidant activity and so on were analyzed and studied.1. Physicochemical characterization:including the moisture content, ash content, conductivity value, pH, free acid content, lactic acid content, total acid, protein content, amylase activity, fructose, glucose, sucrose content of the determination. The results showed that the physical and chemical indexes of honey in Ankang place accorded with the national standard of China and the Codex Alimentarius Commission (CAC) and the national standard of China.2. Selenium content is determined by Selenium content is determined by hydride atomic fluorescence method. The relationship between selenium content and origin in honey was studied. The results showed that the content of selenium in honey was 1.483 ug/kg to 43.326 ug/kg, and the content of selenium in honey was related to the content of selenium in soil, which provided the basis for the development of natural selenium-containing honey products in Ankang.3.The composition and content of free amino acids in honey were analyzed by reversed phase high performance liquid chromatography (HPLC). The results showed that the content of free amino acids in honey was large and full range, the content of total free amino acids was 0.123-2.025 mg/g, the content of various amino acids accounted for no obvious rule. There were significant differences in the composition and content of free amino acids in honey from different regions.4. Take Ankang honey as the research object, the phenolic acid content, DPPH free radical scavenging ability and reducing power were tested in a series of experiments. The results showed that the honey in Ankang has antioxidant activity, especially different levels of antioxidant activity. The DPPH free radical scavenging and reducing power were in direct proportion to the content of phenolic acid in honey, the most important was the strength of both had a dose-dependent relationship. It is to say, the higher the content of phenolic acids, the stronger the DPPH free radical scavenging ability, the stronger the reduction power, and the stronger the antioxidant capacity.