| The artificial insemination(AI)was a key technique of scale breeding on pig,and the semen quality was a key factor to ensure the sow offspring numbers and the success rate of AI.There were three ways used for the preservation of boar semen:normal temperature preservation,hypothermicpreservation,and cryopreservation.The spermatozoa of boar was relatively sensitive to temperature and poor tolerance ability to cold temperature,which had a low cholesterol/phospholipids ofmembrane.Therefore,thenormal temperature preservation of boar semen was still the main way for the production of pig.However,these spermatozoas would produce excessive reactive oxygen species(ROS)during the preservation,which might result in damaging to sperm motility,membrane,and acrosome.Thus it would affect the semen quality during preservation,and reduce the success rate of AI.In order to reduce the ROS damage for spermatozoa,improve the quality of semen preservation.In this study,ellagic acid was added to the Modena extender of boar semen,which was a natural plant extract antioxidant.The results had been detected bycomputer-aided sperm analysis(CASA),fluorescence microscopy,and multi-detection microplate reader.All the data would further benefit the improvement of boar semen quality afternormal temperaturepreservation.The results showed below:1.Different concentrations(0,15,25,35,50,75 μmol/L)of ellagic acid were added into the Modena extender.I had detected the sperm motility,membrane integrity and acrosome intact after preserved 1,3,5,7,9 day,and found that 15,25 μmol/L ellagic acid could weaken the environmental damage,improved the semen quality,but high concentration of EA had opposite effect.Besides,the 7 day’s sperm quality was analyzed: the sperm motility,membrane integrity,and acrosome intact in 25 μmol/L ellagic acid treatment group were significantly higher than other treatment groups(P < 0.05).When the concentration of ellagic acid was 25 μmol/L on boar semen at ambient temperature for 7 days.The sperm motility was 66.63%;the plasma membrane integrity and the acrosome integrity were 84.05% and 87.07%,respectively.Theyshowed significant differences between 25 μmol/L EA and other treatment groups(P< 0.05).The progressive(PRO),average straight line velocity(VSL),and average path velocity(VAP)of spermatozoa were higher than the control group(P< 0.05).In addition,there was no significantly between average curve line velocity(VCL)and the control group(P> 0.05).The results showed that 25 μmol/L ellagic acid could protect the semen.2.The levels of ROS,lipid peroxidation and total antioxidant capacity in spermatozoa were detected atnormal temperature preservation after 7 days.The results showed that ROS and lipid peroxidation were the lowest in 25 μmol/L ellagic acid treatment group,and the total antioxidant capacity was the highest(P< 0.05)compared with the control group.The results indicated that ellagic acid could protect spermatozoa by removing spermatozoa ROS,reduced the level of lipid peroxidation,improved the total antioxidant capacity,and alleviate the oxidation damage during thenormal temperature preservation.3.In the process of cold shock,when the temperature of semen from body temperature to 5℃.It was found that the spermatozoa motility of boars decreased significantly.There were no significant relationships between the different concentrations of ellagic acid and spermatozoa motility after suffered cold shock.The results indicated that ellagic acid couldn’t against cold shock on boar semen.In conclusion,this research indicated that Large White semen supplemented with ellagic acid could scavenge intracellular ROS of spermatozoa,protected the spermatozoa against the oxidation damage,inhibited the decrease of spermatozoa quality during thenormal temperature preservation,and the optimum concentration of ellagic acidwas 25 μmol/L.Meanwhile,the ellagic acid could not preserved the sperm of boar when they suffered from cold shock. |
PDF Full Text Request