Studies On Excretion Of Diaveridine In Chickens And Pigs

Diaveridine is one of the chemically synthesized antibacterial synergist. The combination of DVD and sulfonamide can produce blocking effect at two sites in the metabolic pathway of bacteria folic acid and strengthen the efficacy which can treat coccidiosis and intestinal infection of livestock and poultry.Research focus on the Ecotoxicity of veterinary antibiotics in recent years. The environment veterinary antibiotics were mainly produced by feces excretion of livestock and poultry which can be toxicity to microorganisms in the environment and human. There were few reports about study on residues and excretion of diaveridine in chickens, pigs excreta. The test provide reference in ecotoxicity by developing high performance liquid chromatographic method of Diaveridine residues in excreta of chicken and pigs, and explore excretion regularity of DVD in the chicken feces.The excreta samples were extracted by alkaline ethyl acetate and cleaned up with solid phase extraction columnella of CNW Poly-Sery MCX for analysis. In the excrements of chickens, the calibration curves of DVD was linear in the concentration range of 0.1~50mg/kg, with correlation coefficients more than 0.999.The mean recoveries of Diaveridine ranged from 80.49%~97.60% at the added levels of 0.1, 1, 10mg/kg, with the average intra-batch and inter-batch precisions 1.41%~11.62%. The limit of determination(LOD)and limit of quantitation(LOQ)of Diaveridine in excrements of chickens was 0.05mg/kg and 0.1mg/kg respectively. In the urine of pigs, the calibration curves of DVD was linear in the concentration range of 0.05~5mg/L. The mean recoveries of Diaveridine ranged from 70.57%~96.49% at the added levels of 0.05, 0.5, 5mg/kg, with the average intra-batch and inter-batch precisions 1.27%~16.43%. The LOD and LOQ of Diaveridine in urine was 0.02mg/L and 0.05mg/L respectively. In the feces of pigs, the calibration curves of DVD was linear in the concentration range of 0.1~5mg/kg. The mean recoveries of Diaveridine ranged from 79.19%~95.43% at the added levels of 0.1, 1, 5mg/kg, with the average intra-batch and inter-batch precisions 1.05%~7.31%. The LOD and LOQ of Diaveridine in feces was 0.05mg/kg and 0.1mg/kg respectively. This analytical method meets the requirements for assays.Twenty healthy white feather broiler chickens and twelve duroc crossbred pigs were randomly divided into 2 groups and the blank excreta were collected before administered. Chickens and pigs were feed fodder without DVD before administered. The test 2 groups were administered at a dosage of 10, 20mg/kg of Diaveridine orally. Then the excreta of chickens were collected at 2, 4, 8, 16, 24, 32, 48, 72 h and the excreta of pigs were collected at 4, 8, 12, 24, 48, 72 h, respectively. DVD in the samples were determined by an established method above. The total recovery were 21.63% and 24.13% in chicken excreta at the two dosage respectively. The total recovery were 0.80% and 1.08% in pigs urine at the two dosage respectively. The total recovery were 0.07% and 0.36% pigs feces at the two dosage respectively.The results indicated that prototype drug of diaveridine in chickens and pigs was incompletely excreted, and it probably existed other metabolites and glucuronide conjugates of prototype drug in the excreta. The DVD in chicken excreta excreted fast and more than pigs. DVD was mostly absorbed in the gastrointestinal tract, extensively metabolized and rapid excreted in pigs.