| H. fuscipes is Hemiptera, reduviidae of predatory insects, mainly distributed in Guangdong, Guangxi, Fujian and other Southern tobacco area, preying on cotton leafworm, oriental tobacco budworm, tobacco aphid and other kinds of tobacco pests, it is dominant population of predators of tobacco pests. The development of H. fuscipes experiences three periods: the egg, the nymphal and the adult, after tour ecdyses H. fuscipes from 1instar nymph to 5 instar nymph. The egg stage of H. fuscipes about 7-10d(25℃), the new eggs are light red, and then the color will deeper, they become oxblood red until hatch, the nymphals pierce the lid of egg when hatching; the nymphal stage about 45-50d(25℃), the survival rate of 1-2 instar nymphal is lower, the development time is longer and the body is smaller, they growing rapidly while 3 instar, the body had great changes, the development time had a significant delay in 5 instar; the adult generally 44-54d(25℃), female spawing 2-3 times. There is no relative study about artificial rearing and taxis reaction of H. fuscipes. This paper did a system research of influence of some natural prey and factitious prey on parameters of H. fuscipes and taxis reaction of H. fuscipes on tobacco, cotton leafworm, feces of cotton leafworm. We make a qualitative test for chemical substances by GC-MS, and take use of Y-tube olfactometer to test and finally confirm some semiochemicals can stimulate H. fuscipes to react. By studying artificial rearing, we hope to find an optimal diet and indoor environment to feed H. fuscipes in scale production and industrialization. And by studying taxis reaction of H. fuscipes, we hope to find some semiochemicals can stimulate feeding behavior of H. fuscipes, put this chemical substance into artificial diet and to stimulate feeding of H. fuscipes, to offer material basis of scale production.The contents and results are summarized as follows: 1 influence of some natural preys and factitious diets on growth, development and reproduce of H. fuscipesMeasured influence of some single and mixed diets on growth, development and reproduce of H. fuscipes: larvae of tenebrio molitor, larvae of beet armyworm, pupa of tenebrio molitor, larvae of cotton leafworm and honey. larvae of tenebrio molitor and larvae of beet armyworm are all can meet needs of growth, development and reproduce of H. fuscipes. The development time fed Tenebrio molitor nymph and beet armyworm nymph respectively 48.99±0.47 a and 46.75±0.91 a, no significant difference; the survival rate respectively 77.83%±0.04 a and 85.40%±0.07 b, H. fuscipes fed beet armyworm nymph is higher; preoviposition period of H. fuscipes fed Tenebrio molitor nymph and Spodoptera exigua Hiibner nymph is respecetively 20.15 d and 16.10 d, fecundity is 54.95 and 60.60, weight is 0.077 g and 0.057 g.1-2st H. fuscipes nymph fed with five mixed diet((1)Prodenia litura nymph+ Tenebrio molitor nymph,(2)3d bee+ Tenebrio molitor nymph,(3)6d bee+ Tenebrio molitor nymph,(4)Tenebrio molitor pupa,(5)3d bee+ Prodenia litura nymph), the results show that the survival rate of 1st H. fuscipes nymph respectively is 100%、73.33%、73.33%、63.33%、83.33%,and the survival rate of 1st H. fuscipes nymph respectively is 96.67%、100.00%、100.00%、93.33%、100.00%;the development time of case of(1) 1st H. fuscipes is 7.33±0.60 a,significantly shorter than others; the development time of case and(1)case(5) respectively is 7.00±0.55 ab and 6.44±0.06 a,significantly shorter than others. 2 taxis reaction of H. fuscipes adult on tobacco, larve of cotton leafworm and faeces of cotton leafwormTesting taxis reaction of H. fuscipes adult on tobacco, larve of cotton leafworm and feces of cotton leafworm by a home-made H-device. The result suggests that tobacco has a significant stimulation, while dasheen has a weak result, the larvae of cotton leafworm and the faeces of cotton leafworm has a different stimulation. 3 the extraction and structural identification of cotton leafworm larvae and plant volatilesWe have known chemical substances of cotton leafworm larvae, cotton leafworm faeces, tobacco and dasheen by extraction and GC-MS. There are 15 compounds in Hexane extracts of cotton leafworm larvae: 10 aromatic hydrocarbons, 2 alkanes, 1 alkene, 1 ketone, and one is unascertained. There are 39 comounds in Hexane extracts of cotton leafworm faeces: 11 alkanes, 2 aromatic hydrocarbons, 6 alcohols, 3 acids, 2 amines, 4 alkenes, 2 ketones, 3 esters. There are 52 compounds in Hexane extracts of tobacco: 10 alkanes, 5 alkenes, 3 alcohols, 5 aldehydes, 3 esters, 1 ether, 1 acid. 4 Y-tube Olfactometer for volatile phytochemicalsWith help of Y-tube Olfactometer, we have arrived at a conclusion that just Ethylli nolenate plays a prominent role in attracting H. fuscipes. |
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