Alien Chromatin Analysis Of The Progeny From Octaploid Wheat × Avena Fatua L.

The chromosomic meiotic behavior of pollen mother cells(PMCs), detection and location of alien chromatin in the progeny from wheat(Yi51485) and tetraploid wild oat were carried out by conventional cytology and sequential C-banding- genomic in situ hybridization(GISH) techniques in the present study. The main results were as follows.Cytological analysis showed that a large number of univalents, lagging chromosomes, chromosome bridges, unequal division, asynchronous division, micronuclei and irregular division(three nuclears, 5-8 nuclears) were found at metaphase I, anaphase I, anaphaseⅡand tetrad stages in the F1- F3 progeny. The difference was discovered among three generations, strains or plants. The chromosome number of the progeny was 40-56, the percentage of abnormal cell was 6.67%-96.93%, the meiotic indices were 62.67-93.37, so the progeny were unstable.Sequential C-banding-GISH analysis in the F1- F3 progeny revealed that some small chromosome fragments from the tetraploid wild oat were detected at both metaphase Ⅰand anaphase Ⅰ. The alien chromatin was located in lightly stained chromosomes, which belonged to the genomes A(1A, 3A, 4A and 7A) or D(1D, 2D, 3D and 6D) of Yi51485. Partial alien chromatin originated from the tetraploid wild oat might get lost in the genetic transmission, so the progeny became relatively stable.The chromosomic behavior and constitution of the progeny from wheat Yi51485 and the tetraploid wild oat were understund by the analyses of PMCs meiosis and GISH in the present study.