Preparation Of Anode For Microbial Fuel Cell And Detection For Gentamicin

Microbial fuel cells(MFCs)are bioelectronic devices that convert the chemical energy into electricity from the living microorganisms.As a novel kind of biosensor,MFCs have attracted much attention in the research fields of environment,food engineering and medical science that attribute some advantages to simple structure,low cost and environment friendly.However,their low output power has limited their biosensor performance.How to improve the performance of MFCs is a prime problem.The anode plays a key role in MFC that directly contact with electricigens.The performance of MFCs mainly depend on the electron transfer rate that is obviously affected by the material and structure of anode,which decide the amount of bacteria,the rate of electron transfer and the level of oxidation on the anode.Therefore,we modify ITO anode with carbon nanotubes and conductive polymers by layer-by-layer(LBL)technique in order to improve the performance of MFC.Subsequently,we choose the best performance of anode to build a biosensor based on MFC for detecting the antibiotics.The main work of this paper is as follows:1.Modify the ITO electrode with CNTs(carbon nanotubes)/PAH(polyene propyl amine salt)based on LBL technology to improve the specific surface area of the anode.Experimental results of performance have shown that the current density increased from 0.266 ?A/cm~2 to 3.87 ?A/cm~2 and the power density increased from 4.63 m W/m2 to 5.98 m W/m2,which can availably improve the electricity production of Shewanella lohica PV-4.2.CNTs/PANI multilayer films modified ITO electrodes are fabricated by LBL technique.The modified layer numbers effect on the performance of MFCs was studied.The reults shows that the maximum current density obtained as the layer increased to 12.The power density was enhanced 29.1% than without modified anode.3.Basic study of the biological properties of E.coli 25922,and find out a suitable time for bacteria cultivation and current testing.The biosensor was build with(CNTs/PANI)12-ITO anode based on MFC.The results show that the different concentrations of gentamicin can be detected by the biosensor immediately.The limit of detection is 0.25 m M.