Preparation,characterization And Sensors Research Of Fluorescence Nanomaterial

Diabetes,cancer and cardiovascular disease are the three common global diseases,which seriously threaten human health.The diagnosis and treatment of diabetes is one of the most important research topics with more and more attentions gained among the researchers in recent years.Nanoparticle becomes an ideal fluorescent probe which plays a key role in the diagnosis and treatment of diseases due to its unique luminescent properties,low toxicity and superior pharmacokinetic properties.However,the surface inert of the AuNPs becomes a drawback when looking for luminescence intensity response based sensing applications.Therefore,the establishment of a novel approach based on the luminescence quenching of the AuNPs is very important in the field of biosensor applications.Hydrogen peroxide?H₂O₂?is the main intermediate and product of many enzyme catalytic reaction,it plays a key role in the process of the biosynthesis and cell label.TCEP is a strong reducing agent that reduces disulfide and hydrogen peroxide rapidly and stoichiometrically in solution.Besides its reduction ability,TCEP is also a polydentate and hard-soft donor ligand for metal coordination,in which the phosphine moiety can bind to low-valent metal ions to interact with the metal NPs.Herein,the study of the interactions between biologically reducing and coordinating reagent TCEP and luminescent AuNPs are of great importance due to the potential optical biosensor applications.Therefore,we report two novel analysis detection approach to induce significant luminescence quenching of the AuNPs for the detection of H₂O₂ and TCEP,the visible and near-infrared?NIR?luminescent AuNPs with core size below 3 nm was synthesized by metal-ligand?Au-S?.We can discovery the binding between Au?I?of GS-AuNPs and P atom of TCEP,which lead to the luminescent of AuNPs.On the other hand,TCEP can be rapidly and stoichiometrically oxidized by H₂O₂,hence,it is possible to construct an analytical method for the determination of H₂O₂ based on the above GS-AuNPs and TCEP system.In addition,glucose oxidase?GOD?can catalyze the oxidation of glucose to H₂O₂ and gluconolactone with dissolved O2,as a result,it was very convenient to detect glucose based on monitoring the production of H₂O₂,the limit of detection?LOD?of the visible luminescent AuNPs is 0.37 nM and 5.40 ?M for H₂O₂,respectively,the limit of detection of NIR luminescent AuNPs is 1.74 ?M and 0.42 ?M for glucose,respectively.The highly extendable feature of this detection strategy can have great potential in the fabrication of other types of biosensors relevant with the product of H₂O₂,such as the detection of cholesterol and urine acid catalyzed by the their enzymes.