Design,Synthesis And Binding Ability Of Fluorescent Probe For Cyanide Base On Fluorescence Resonance Energy Transfer

Anions are widely present in the human body and natural environment,and which also play an important role in many chemical and biological processes.Therefore,the design and development of novel artificial synthetic anion fluorescent probes has attracted much attention in supramolecular chemistry.Based on the FRET mechanism,in this thesis,a novel probe B6 based on ICT-PET-FRET and its reference probe A6 are designed and synthesized for the first time.Compared to the single binding of CH group in the reference probe A6,probe B6 contains the double binding sites NH and CH groups.The potential mechanism of probe B6 is anion-CH group hydrogen bonding induced ICT followed by the corresponding anion-NH group hydrogen bonding induced PET,and further the corresponding FRET processes between coumarin and naphthalimide fluorophores was also constructed.It shows that B6 has high selectivity and competition than A6 in the UV-vis and fluorescence spectra.The UV-vis detection limits of probes A6 or B6 was determined to be 1.10?M and 6.91?M,respectively,and the corresponding fluorescence detection limits was 0.52?M and 0.11 ?M.The addition of cyanide ions,the UV-vis spectrum of A6 has a large red-shift about 311 nm and the fluorescence spectrum of A6 was quenched turn-off,and that of corresponding B6 has a red-shift about 281 nm and a specific emission response(on-off-on)to the CN-in THF was observed,which indicates that the dual binding site of CH and NH group in probe B6 showsmore advantages than A6.In addition,two receptors would also work in the solid state for detecting CN-with moderate different fluorescence color,therefore,it can be developed for potential test paper applied in the environments.