The Separation And Purification Of Rice Bran Protein Peptide ACE

To effectively improve rice bran added value,increase rice bran product,this experiment with rice bran protein as raw material preparation rice bran ACE irus-inhibitory peptide.Using ultrafiltration macroporous adsorption resin SephadexG-15 glucan gel chromatography for separation of ACE inhibitory peptides from rice bran.The result was highly active ACE inhibitory peptides from rice bran.After the separation of the components that make use of high performance liquid chromatography(HPLC)method to analysis and research of ACE activity.The main research contents and conclusions are as follows.1.Through enzyme screening test to determine the compound protease enzyme of the first one.The rest of the four kinds of enzyme for position as the second kind of hydrolytic enzymes.Research results show that the alkaline protease hydrolysis+flavor protease substrate concentration 5%,through L9(3~4)orthogonal test to get the best optimum combination,the results for temperature50?for hydrolysis,p H 10.0,hydrolysis time2.5h,enzyme usage is[E]:[S]?3.0:100,In these conditions rice bran ACE antihypertensive peptides inhibition of86.52%.2.First choose ultrafiltration membrane separation and purification of 1000u and 3000u to intercept molecular weight of rice bran protein hydrolysate,after after the ultrafiltration of rice bran protein hydrolyzate of ACE inhibitory activity than before ultrafiltration has improved significantly.3.Developed a method to separate Rice bran ACE inhibition peptide with macroporous adsorption resin,The results showed that the HPD-400 type resin was the most suitable in the rice bran protein peptide.The influence of various factors on the resin adsorption and desorption,process parameters..Static adsorption of 4h HPD-400 resin for rice bran polypeptide of about 45?to reach adsorption equilibrium,adsorption temperature is appropriate;adsorption time was over 2 h type HPD-400 resin for rice bran protein peptide adsorption capacity has already reached saturation;when pH 4 adsorption effect is good,the adsorption rate of85.4%.Desorption solution for p H 8 and 70%ethanol solution.The elution time for determining the liquid30min.HPD400 resin before and after the separation of ACE inhibitory rate increased from 85.67%to 91.76%,salt content changes significantly reduced to 1.80%from 19.9%before resin HPD400 desalination.4.Experimental study the Sephadex G-15 separation of rice bran protein hydrolysate,comprehensive analysis,with deionized water as eluent,flow rate of 0.8 mL/min,sample concentration in 200 mg/m L,the best separation effect.After separation of components by high performance liquid chromatography(HPLC)method,through the molecular weight of standard curve to determine the molecular weight of separating the active component of the molecular weight range from 730~990 u,ACE inhibitory rate reached 92.5%.5.Experimental study on the heating temperature,p H,gastric,pancreatic curd protease enzyme in rice bran ACE experiments the influence of active peptide,the test results show that the temperature at 0?,pH8ACE inhibition rate is highest,highly active ACE inhibitory peptides from rice bran can keep high activity in the gastrointestinal tract.