Discrimination And Identification Of Proteins And Microorganisms By Fluorescent Array Sensor

Detections of biological macromolecules such as proteins and microorganisms are quite necessary in medical diagnosis. From this point of view, it is of great importance to develop novel methods for the efficient and rapid detection of proteins and microorganisms. The array sensor is an attractive approach in which the functions of multi sensors are integrated, offering the merits of high resolution and short detection time. The aim of this work is to construct a fluorescent array sensor for the discrimination and identification of proteins and microorganisms.In chapter 1, brief description is given to the research background and corresponding progress and development of array sensor. In addition, the properties, synthesis and applications of ionic liquids and quantum dots are also briefly introduced.In chapter 2, the performances of fluorescent array sensor based on 5 types of ILs in the discrimination of 8 target proteins are firstly studied. A discrimination accuracy of 46/48 is achieved under a protein concentration of 1000 nmol L-1, and the discrimination accuracy deteriorates with the decrease of protein concentrations. In order to improve the the performances of the array sensor, fluorescent sensor array based on ILs-CdTe QDs/CdTe QDs are further constructed. The experimental results indicate that the ability of the newly developed sensor array to the discrimination of 8 target proteins has been significantly improved. At a protein concentration as low as 10 nmol L-1, the discrimination accuracy is 100%. Conclusively, this fluorescent array sensor could effectively discriminate 8 proteins at same concentration of as low as 10 nmol L-1, or a specific protein at various concentrations within a range of 10-5000 nmol L-1, or 2 proteins with different concentrations, or a mixture of two proteins at various proportions. These results well illustrated the potentials of the fluorescent array sensor in protein discrimination.In chapter 3, the identification of 6 target microorganisms is achieved by using QDs-based fluorescent array sensor, based on the nonspecific interactions between microorganisms and ILs-CdTe QDs/CdTe QDs. The experimental results indicate that the accuracy of the identification is 90% at a OD600 value of microorganisms at 0.1. When the concentration of the microorganisms go down to OD600=0.1/10, an accuracy rate of 80%　is still achieved. These results indicated that the fluorescent array sensor might be used to identify microorganisms with different proteins.