Oxidation Of2-chlorophenol Catalyzed By Two Water-soluble Metal Phthalocyanines And Its Optical Detection

Chlorophenols are the common pollutants in environment. Because of their hightoxicity, difficulty of biological degradation and ease of diffusion, whenchlorophenols enter the environment they can cause serious pollution to it. Therefore,it is of great importance to the human health and environment protection to develop afast and effective detecting method for the detection of chlorophenol pollutants. Thefiber optic sensors based on enzyme catalysis have many advantages such as highdetecting precision, strong ability to resist disturbance and possibility of realizingreal-time and online detection, and can be the one of effective ways to detectchlorophenols. Metal phthalocyanine (MPc) is a planar ring-conjugated complex withoutstanding performance of biomimetic catalysis and excellent stability, and thepreparation of water soluable MPc will be helpful to the catalysis to the chlorophenoloxidation, which will have great application prospect in the field of chlorophenoldetection. Quantum dots (QDs) can be used as the optical sensing materials due totheir advantages of high fluorescence quantum yield, high brightness, good opticalstability, and adjustable emission wavelength. In this thesis, two water soluble MPc,FePc-(QnSO3H)4and Fe(III)PcTs, were prepared. The fiber optic2-CP sensor basedon FePc-(QnSO3H)4catalysis has been fabricated and studied. The fluorescencedetecting method for2-CP based on Fe(III)PcTs–CdTe QDs system has been studied.Satisfying results have been obtained for both of them.A new kind of water soluble MPc, FePc-(QnSO3H)4），has been desighed andprepared. With4-AAP as the chromogenic agent, FePc-(QnSO3H)4）can catalyzefastly the oxidation of5chlorophenols (phenol,2-CP, DCP,4-CP,1-NP) to form thepink quinone imide dyes. The dye formation rates for these five chlorophenols are inthe following order: DCP>2-CP>Phenol>4-CP>1-NP. The best pH for this catalysisoxidation is7.0and the best raction temperation is25℃；A fiber optic2-CP sensor based on FePc-(QnSO3H)4catalysis has beenfabricated. The fluorescence indicator Ru(bpy)3Cl2was used as oxygen sensingindicator to prepare the optical oxygen sensing film which was immobilized on thesensor head. The2-CP concentration was detected by detecting the change of oxygen concentration in the system. In the2-CP concentration range of1.0×10-6mol/L~9.0×10-6mol/L and1.0×10-5~9.0×10-5mol/L, the2-CP concentration has good linearrelationship with the relative phase delay of the sensor head. The detection limit ofthe sensor is1.0×10-6mol/L and its response time is15min. This sensor has goodrepeatability and long-term stability.Another water soluble MPc, Fe(III)PcTs has been prepared. With the catalysis ofFe(III)PcTs, BuOOH could oxidize2-CP to form quinone imide dye which couldquench the fluorescence of CdTe QDs. With the CdTe QDs as the fluorescence probe,2-CP detection system based on Fe(III)PcTs catalysis was fabricated. In the2-CPconcentration range of1.0×10-6~1.0×10-5mol/L, P0/P, the quenching degree of CdTeQDs fluorescence, has good linear relationship with2-CP concentration, and itsdetection limit is1.0×10-6mol/L. This detecting system has good repeatability andstrong ability to resist disturbance. Satisfying results were obtained for the detectionof real sample using this system, showing the application potential of this system.