| [OBJECTS] Structure "high-fat high-sugar+streptozotocin (STZ)"-induced diabetic rat model to explore the treatment of diabetic rats Dendrobium mixture of the molecular mechanism.[METHODS]PART 1:Selected Wistar rats, male and female in half, body mass 130g±20g/were randomly divided into normal (A) group and model group (B). Making module (B) use of high-fat high-sugar feeding 4 weeks after the intraperitoneal injection of STZ was prepared by diabetes mellitus (DM) rats to fasting blood glucose (FBG)≥11.1mmol/L into a mold for the standard, statistics into a mold rate, mortality rate.PART 2:Use the first part of DM rats (B group), to enter drug treatment, were randomly divided into two groups:4w (B4) Group,8w (B8) group. Each group was randomly divided into model control group and Dendrobium mixture treatment group; 4w (B4) Group & P Fresh feeding 4w, drug treatment 4w; 8w (B8) Group & P food feeding 8w, drug treatment 8w. Then test indicators, blood glucose meters to detect FBG, abdominal aortic blood, random serum glucose, insulin, glycosylated serum proteins, lipids, etc.; take pancreatic tissue, HE stainingobservation of routine biopsy of pancreatic structure; reverse transcription-polymerase chain reaction (RT-PCR) to detect PDX-1, GLP-1R, FOXol, FOXa2 expression..PART 3:Finishing phases, group results, statistical analysis[RESULTS]PART 1:Into a mold, mortality results showed that:the composition of model modeling the rate of 97.92%, mortality rate of 0.PART 2:1 Blood biochemistry showed:For the FBG, horizontal comparison, both 4w or 8w, DC treatment group were lower than the model group (P all<0.01), vertical comparison, both the model group or the DC group,8w lower than those of 4w (P all<0.01). For insulin, horizontal comparison, both 4w or 8w of the DC group was significantly higher than the model group (P all<0.01), vertical comparison, DC group than in 8w in 4w low (P<0.05). For the glycosylated serum protein, DC group (4w and 8w) than the model group also decreased significantly (P<0.05), cholesterol, triglycerides DC group (4w and 8w) lower than the model group (P all<0.01).2 pathological Show:horizontal comparison, whether it is 4w or 8w, compared with the model control group, DC group significantly increased the number of islets and islet area is significantly increased; vertical comparison with 4wDC group,8wDC group should be more than the number of islets, insulin slightly larger in area. However, compared with the normal control group, DC group (4w and 8w) islet number slightly less than the normal control group, islet size is also slightly smaller.3 RT-PCR assay results showed that:horizontal comparison, the control group compared with the model, whether it is 4w or 8w, DC group of PDX-1, GLP-1R, FOXa2 expression is clearly higher expression of the FOXo1 much lower, vertical comparison, the 8w DC group of PDX-1, FOXa2 higher expression than in 4w, and GLP-1R, FOXol expression between the two groups was not statistically significant.[CONCLUTION]1 High-fat high-sugarModeling method achieved high modeling rate(97.92%) and 0 death rate.the DM model rat was of feasibility.2 DC could decrease the level of blood glucose, TG,TC, and the closely related with the DC treatment cycles (8w than 4w better), DC can promote insulin secretion (compared with model group), lower glycosylated serum protein, to promote regeneration of pancreatic 6-cell gene expression of PDX-1, reduce FOXol inhibit GLP-1 to promote pancreaticβcell proliferation and anti-apoptotic role.3 DC possible mechanisms:①DC increased the expression of GLP-1.②GLP-1 expression increased and then more easily to its receptor, which initiates downstream signaling.③DC inhibited the expression of FOXol strengthen FOXa2 expression.④inactivated FOXol can not compete with FOXa2 PDX-1 binding to the promoter, thereby inhibiting loss of the capacity of PDX-1 transcription.⑤DC to promote the expression of PDX-1mRNA.⑥PDX-1 transcription strengthened, so as to promoteβ-cell proliferation, enhance insulin secretion. |
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