Study On Several Kinds Of Traditional Chinese Medicines By Infrared Spectroscopy And X-ray Diffraction Analysis

The necessity of whole quality evaluation of Traditional Chinese Medicine(TCM) is decided by its complexity and whole medication. Infrared spectroscopy and X-ray diffraction analysis based on the identification of TCM are effective analysis methods which can reflect the whole characteristics of TCM quality. The establishment and extension of control infrared spectra and control X-ray diffraction patterns will make great contribution to the establishment of quality control standard of TCM.The qualitative identification of Chinese Herbal Medicine(CHM) and Chinese Patent Medicine(CPM) was investigated in this thesis with infrared spectroscopy and X-ray diffraction technique and the reliable experimental data could be provided for the establishment of fingerprint of TCM. The dissertation consists of four chapters.In Chapter One, the recent progress of application of Infrared spectroscopy and X-ray diffraction analysis in TCM was reviewed with 73 references. The objective and significance of this dissertation were described in brief.In Chapter Two, Rhizoma chuanxiong was investigated by infrared spectroscopy and the experimental conditions which affected the infrared spectra of solid powder and extraction of Rhizoma chuanxiong were studied. The infrared spectra of solid powder and the hexane, ether and methanol extractions of 20 Rhizoma chuanxiong samples were determined under the optimum experimental conditions and the control infrared spectra were established using mean value method, respectively. The results showed that the advantage aspects of the proposed method were good precision, repeatability and stability. This work made a basis for the establishment of control infrared spectra of other TCMs.In Chapter Three, the qualitative identifications of 8 kinds of umbelliferae CHMs were carried out using infrared spectroscopy and X-ray diffraction technique, respectively. The Radix Glehniae and Radix Angelicae pubescentis were identified using solid powder second derivative infrared spectra. Using normal and second derivative infrared spectra of hexahydrobenzene, petroleum ether, ethyl acetate and hexane extractions, the Rhizoma chuanxiong and Rhizoma Ligustici, Radix Bupleuri and Rhizoma Seu radix notopterygii could be identified, respectively. The Radix Bupleuri, Rhizoma Ligustici, Rhizoma Seu radix notopterygii and Radix Glehniae could be identified with second derivative infrared spectra of acetone extraction and the Rhizoma chuanxiong, Radix Peucedani, Radix Angelica sinensis, Radix Angelicae pubescentis and Radix Glehniae could be identified using the second derivative infrared spectra of ether extraction. The significant differences were observed in the diffraction patterns of 8 kinds of umbelliferae CHMs with similarity degrees, t-test results of similarity degrees and the comparison of diffraction peaks. The results showed the X-ray diffraction could be used for the identification of 8 kinds of umbelliferae CHMs for its good precision, repeatability and stability.In Chapter Four, the infrared spectra and X-ray diffraction patterns of 7 kinds of CPMs were investigated. Effect of several experimental conditions including particle size, mixing ratio and scan times on the infrared spectrum of Suxiao Jiuxin Wan were studied. The infrared spectra of Suxiao Jiuxin Wan with the same and different batch numbers from the same manufacturer were determined and the results showed that the quality of Suxiao Jiuxin Wan from same manufacturer had good uniform and stability. The significant differences of quality were observed between the infrared spectra and x-ray diffraction patterns of 2 Suxiao Jiuxin Wan samples from different manufacturers, respectively. The infrared control spectra of 7 kinds of CPMs were established using mean value method under the optimum conditions, and the significant differences were found between two groups by comparison of the second derivative infrared spectra of 7 kinds of CPMs. The experimental results of X-ray diffraction of the 7 kinds of CPMs showed that there were obvious differences in X-ray diffraction patterns including geometric topology rules, position and intensity of diffraction peak. The experimental results showed that the second derivative infrared spectra and X-ray diffraction patterns could be successfully used for the qualitative identification of the 7 kinds of CPMs.