This article embarks from coptidis rhizome’s by-product,through carries on the determination to its by-product effective component content,determines take the whisker as the finally object to study.Uses each kind of alkaloid monomer content and compound total alkaloid content which many kinds of method determination the whisker coptidis rhizome。Through the urgent toxicity test,the long-term toxicity test and so on have carried on the preliminary secure appraisal.Through to gives off heat of the big mouses to allay a fever the function proof to have the refrigeration function,kata-blood fats function through the animal model to it to conduct the research,tests the result to be as follows specifically:.(1 According to the pharmacopoeia method determination coptidis rhizoma beard hit the total alkaloid and each alkaloid monomer content:The total alkaloid 2.54%,jateorhizine 0.376%,coptisine 1.165%,palmatine 0.071%,berberine 0.935%.(2 In order to save the cost to use the acid water extraction,the cellulase extraction,the supersonic extraction,the microwave oven extraction separately and so on the different extraction method,discovered withdraw 24 hours effects to be best which 0.3%sulfuric acid water-soluble fluid.(3 the whisker coptidis rhizome extract through the D101 resin has carried on the decolorization,the purification to its compound total alkaloid.The full use compound total alkaloid oversaturation adsorption principle,obtains the compound total alkaloid purity to reach 81.5%.(4 the whisker of coptidis rhizome hit the compound total alkaloid to the coptidis rhizome which withdraws to carry on the secure appraisal.The urgent toxic experiment discovers LD50>7500mg/kg,according to the compound toxicity graduation standard,is the non-toxic level.Long-term toxicity test discovery mouse performance not abnormal phenomenon.Also discovers the not abnormal phenomenon when occurrence to its dissection.(5 Through to inject the big mouse to the barm for give offheat the model to allay a fever the experiment,uses the digital thermometer determination coptidis rhizoma to have to hit withdraws the compound total alkaloid and does not dissolve the water material to fill the stomach big mouse anus warm change experiment,discovered the whisker coptidis rhizoma hit withdraws does not dissolve the water part to have the refrigeration function,also the effect is obvious.(6) coptidis rhizoma hit the compound total alkaloid which withdraws to be possible to reduce the high fat feed to send hyperlipemia mouse TC,TG,LDL-C,elevates LDL-C, the suppression fat in the abdominal cavity and liver concentrating,has good reduces the blood fats function,also by 1:1 blends the effect with the Chinese hawthorn brass to be also remarkable. |