| There are abundant resources of marine algae in China, and fucoidan in brown algae as kelp has become a hotspot research in current years. Many studies have shown that fucoidan has some effects such as anti-oxidation, anti-thrombotic and anti-tumor. The separation and purification technology of fucoidan is so immature that the exploitation of fucoidan is limited. Currently,the cost of separation and purification fucoidan is high, at the same time there are a large amount of wast er water discharge. Here in, the separation and purification of fucoidan was studied and the main contents are as follows:This study was based on the phenol-sulfuric acid method for the detection of fucoidan, the optimum reagent ratio was 2:0.5:6(raw material / phenol / sulfuric acid), and color reaction was steady within 25 min at 80℃. Based on the adsorption and enrichment characteristics of brown algae to metal ions, the edible and medicinal safety was studied. Inductively Coupled Plasma-Atomic Emission Spectroscopy(ICP-AES) was established to analysis the metal ions in the sporoph yll of undaria pinnatifida suringar, kelp and their leaching residue after drying. We found that the content of Fe, Cu and Zn were 0.1% less than the standard of trace elements, and Co and Ni below the detection limit, so the brown algae could not do harm to people and were edible and medicinal.In this study, fat-soluble substances such as pigments in sporophyll of undaria pinnatifida suringar and kelp to be leached, which not only obtains the pigments and other by-products, but also avoids the affecting of further separation of fucoidan. Diacolation effect of sporophyll of undaria pinnatifida suringar in moisture content of 10.21% and particle size of 80 mesh with 90% of ethanol at a flow rate o f percolation effect of 0.33 ml/min is relatively good, the first leaching rate is 79.43%. And diacolation effect of kelp in moisture content of 8.83% and particle size of 120 mesh with 85% ethanol at a flow rate of percolation effect of 0.33 ml/min is relatively good, the first leaching rate is 68.75%.We used non-toxic or low toxic extraction agent to extracting fucoidan from kelp fermentation by solvent extraction. Carried on the single factor experiment to the better system N404 and A103 and the results are as follows: th e extraction temperature of N404 was 25℃, the ratio was 2:1, the p H of raw material solution was 0.20, the rotation speed was 500 r/min, the extraction time was 5 min, the extraction rate of fucoidan is 24.49%; the extraction temperature of A103 was 25℃, the ratio was 1:1, the p H of raw material solution was 1.00, the rotation speed was 500 r/min, the extraction time was 15 min, the extraction rate of fucoidan is 24.38%. The N404 system with higher extraction rate was chosen for orthogonal experiment, and the conditional temperature of 35℃, ratio of 3:1, the raw material solution p H of 0.05, rotation speed of 400 r/min, and extraction time of 7 min were determined, the extraction rate of fucoidan is 30.13%.The dynamics of pigment extraction and thermodynamics of fucoidan extraction was discussed in this study. The dynamics model of undaria pinnatifida sporophyll extraction is R2 = 0.9805 and the dynamics model of kelp is R2 = 0.9736. It indicates that the mass transfer process is controled by the diffusion. The △Hm of fucoidan in N404 and A103 extraction system is-5.73 k J/mol and-6.11 k J/mol, respectively. Since the reaction of extraction is exothermic, low temperature is beneficial to the process of extraction. The result of IR show that fucoidan has been extracted into the organic phase. |
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