The Effect Of Phospholipid Transfer Protein On Cigarette Smoke Extract Induced Epithelial-mesenchymal Transition Of RLE-6TN Cells

Objective:To investigate the effect of phospholipid transfer protein(PLTP) on cigarette smoke extract(CSE) induced epithelial-mesenchymal transition(EMT) in rat alveolar Type II cells(RLE-6TN).Methods: The different concentrations of CSE co-cultured with RLE-6TN for 2 or 3 days; cells were either pre-treated or not pre-treated with PLTP-si RNA for 6 h. Expression levels of E-cadherin m RNA and Vimentin m RNA were examined by RT-PCR; Expression levels of PLTP,E-cadherin,N-cadherin and Vimentin were examined by Western blot.Results: Our results showed that the expression of E-cadherin m RNA decreased in CSE-treated groups:(1.01±0.05),(0.74±0.05),(0.65±0.03),(0.30±0.08) respectively;and expression levels of Vimentin m RNA obviously increased in 1%, compared with control group:(1.88±0.49) VS(1.01±0.20); Protein levels of PLTP were(0.42±0.02),(0.89±0.25),(1.08±0.18),(1.61±0.06) respectively; E-cadherin were(1.61±0.04),(1.08±0.10),(0.62±0.08),(0.68±0.17); N-cadherin were(0.60±0.14),(0.57±0.26),(0.88±0.30),(1.94±0.54); Vimentin were(0.61±0.05),(0.98±0.16),(1.07±0.14),(1.34±0.19). all of 1% P<0.05 as compared with control group. EMT induced by CSE is significantly inhibited by PLTP-si RNA. Conclusion:1 CSE could activate PLTP to induce EMT of alveolar epithelial cells. 2 PLTP-si RNA could knock down the expression of PLTP, and partly reverse the CSE induced EMT. So PLTP may be involved in the CSE induced EMT of rat alveolar cells.