The Effects Of LKB1 Gene Down-regulation Induced By ShRNA On Expression Of CyclinD1、Mcm2 And PCNA In Prostate Carcinoma Cell

Objective: To investigate the effect of LKB1 gene silencing on expression of cyclinD1、Mcm2 and PCNA of human prostate carcinoma PC-3 cells as well as the relevant mechanism..Methods: The shRNA expression plasmid of LKB1(LKB1-shRNA1)was constructed and transfected to PC-3 cells by liposome-mediated transfection, while using the cells untransfected as blank group and those transfected with Vector as negative group, and the well-transfected cells were screened out using G418.The transcription levels of LKB1,cyclinD1,Mcm2 and PCNA mRNAs in the transfected cells were determined by RT-PCR, while the expression levels of LKB1,cyclinD1,Mcm2 and PCNA proteins by Western blot. Testing after transfection cell proliferation ability by CCK-8.Results: Compared with those of blank and shNC groups, the relative levels of LKB1,cyclinD1,Mcm2 and PCNA ’ s mRNA and proteins in LKB1-shRNA1 cells increased significantly(P<0.01)Conclusion: Gene silencing of LKB1 could up-regulate the expressionof cyclinD1、Mcm2 and PCNA in prostate carcinoma cells; LKB1 gene might be a potential target for study on the molecular mechanism of proliferation of prostate carcinoma.