Study Of Fuzhenghayukangxian Decoction On The Expression Of TGF-β1 And PDGF Of Hepatic Fibrosis Rats

Objective:The research by carbon tetrachioride method to establish animal model of hepatic fibrosis in rats,Fu Zheng Hua Yu Kang Xian Decoction on rats of intervention,which observed liver tissue structure changes though light microscope in a rat models.And made use of Western blot、PT-PCR detecting TGF-β1,PDGFto explore the possible mechanism of Fu Zheng Hua Yu Kang Xian Decoction in prevention and treatment of liver fibrosis.Methods:15 in 70 Wistar rats were randomized into blank control group,model control group,Fu Zheng Hua Yu Kang Xian Decoction of high,medium and low doss groups,positive control groups.except the blank control group has 10 rats,12 rats in rest each group,Rat hepatic fibrosis model was made by compound factors,the blank control group with normal feed and water,the rest of the group by normal diet,with 10% edible alcohol as the only beverage and intraperitoneal injection of 40% CCL4 olive oil agent(for the first time with 5ml/kg,after every three days injection of 3ml/kg),2times/week,for 6 weeks,Starting from the first day of the experiment,ursodeoxy cholic acid to drug 0.54g/kg gavage; Fu Zheng Hua Yu Kang Xian Decoction group was given medicine high dose 18.9g/kg,middle dose 9.45g/kg,low dose 4.7g/kg irrigation stomach;blank group and model group were given normal saline 0.54g/kg gavage.After6 weeks treatment materials.All rats using femoral artery bleeding,along the mesenteric longitudinal sectioned,rat liver,saline rinse,observation of general morphology,then clipping the right lobe of the liver tissue 3mm×2mm×3mm, fixed in 10% formaldehyde solution, after dehydration, paraffin embedded, section,hematoxyclin and eoxsin, to observe the morphological changes of liver tissue, and Western blot was used to observe the liver tissue TGF and PDGF expression, RT-PCR detection of liver tissue TGF beta 1 m RNA and PDGF m RNA.Results: 1 The rat hepatic fibrosis model was established by modeling the intervention for6 weeks, and the control group compared to the model group rats liver color dark red visible yellow flaky patches, the texture is hard, margin obtusely. treatment groups rats liver have different degrees of yellow spots, the edge is blunt, but superior to that of the model group,Nakedeye observation of liver changes in the treatment group, the lightest in traditional Chinese medicine high dose group of liver fibrosis than western medicine group, Chinese medicine dose group fibrosis more serious than western medicine group, low dose of Chinese medicine group has the most serious performance of fibrosis; tissue sections with hematoxylin and eosin(HE),model group liver cell swelling, degeneration and necrosis and extensive fiber hyperplasia;treatment group sections of rat liver tissue inflammatory pathological changes have different degree of loss, fibrous tissue proliferation. It is suggested that in the model group and the treatment groups were made model, traditional Chinese medicine group and Western medicine group can be used to liver fibrosis development to a certain therapeutic effect.2 Before the intervention of body weight of rats and 6 weeks after the intervention of the body weight of rats were compared. After the intervention of rat body weight in varying degrees of growth, the body weight of the rats in the model group growth slow(P < 0.05), model group and the treatment group was compared in the treatment group, the body weight increased, to traditional Chinese medicine high dose group and Western medicine group, the weight growth obvious(P < 0.01), middle dose group and low dose group, the body weight growth changes had no significant difference(P > 0.05).3 Hepatic fibrosis in rat liver tissue TGF beta 1 and PDGF in Western blot were used to detect the expression: in the blank group, TGF beta 1, PDGF have a small amount of protein expression, compared with the blank group, model group, rat liver tissue TGF beta 1 and expression of PDGF increased significantly(P < 0.05), and model group than traditional Chinese medicine in the treatment group, western medicine group in TGF beta 1 PDGF expression are decreased(P < 0.05), prompt interventions are effective. The expression of the Chinese medicine high dose TGF beta 1, PDGF decreased significantly(P < 0.01).Traditional Chinese medicine low dose group treatment effect is inferior to the rest of the treatment group, but better than the model group(P<0.05).The expression of TGF- beta 1 in Chinese herbal medicine group and Western medicine group were decreased(P<0.05), and there was no significant difference between the two groups(P>0.05).and PDGF in traditional Chinese medicine high, medium dosegroup is better than western medicine group(P<0.05).4 RT-PCR detection of liver tissue TGF beta 1 m RNA and PDGF m RNA expression with the blank group compared to model group, rat liver tissue TGF- 1 and PDGF expression as compared to normal tissue increased significantly(P < 0.01), in the treatment group, the expression of value were decreased(P < 0.05), the intervention is effective, the traditional Chinese medicine high dose group of TGF beta 1 expression decreased significantly(P < 0.01),the dose group and Western medicine group expression equivalent, compared with no significant difference(P > 0.05), western medicine group was superior to the traditional Chinese medicine low dose group(P < 0.05); PDGF Expression in high dose of Chinese medicine group and Western medicine group were significantly decreased(P < 0.01), but the expression between the two groups value had no significant difference(P > 0.05); in the low dose of Chinese medicine group was despite the decline, but curative effect is inferior to the rest of the treatment group(P <0.05).Conclusion: 1rat by carbon tetrachloride method intervention 6 weeks may induce typical models of liver fibrosis; 2Fu Zheng Hua Yu Kang Xian Decoction high dose group can significantly reduce the liver tissue of rats with hepatic fibrosis transforming growth factor the expression of TGF beta 1, PDGF, reduce rat liver injury and fibrosis, the mechanism may be related to the inhibition caused by fiber cytokine the expression of TGF beta 1,PDGF.