Effects Of Fecal Microbiota Transplantation On Lipidemia And Gut Barrier In Diet-induced Obesity Rats

Objective:To investigate the protective effect of fecal microbiota transplantation(FMT) on lipidemia, gut barrier and liver in rats with diet-induced obesity.Methods:1.Forty SD male rats were randomly divided into three groups:one normal control group(NG group;fed a normal diet for 6 weeks),two high-fat diet groups(HGA and HGB groups;fed a high-fat diet for 6 weeks).HGA group recieved fecal microbiota transplantation;The rats in NG and HGB group were given normal saline enema,once a week,continuous enema administration for 3 weeks.2.Changes in the body weight and Lee index were measured at the end of the sixth-and ninth-week.At the end of ninth-week, the concentration of endotoxin、TNF-α、TC and TG in blood were detected.Liver index were measured and the liver tissue samples were collected for pathology examination.3.The statistical analysis was done through SPSS13.0 software,measurement data were indicated with the mean±standard deviation,The statistical significance of differences was analyzed by one-way ANOVA. Comparison between two groups using LSD test.P<0.05 for the difference was statistically significant.Results:1.Diet-induced obesity rat was successfully established. The body weight of high-fat group significantly increased in the during the modeling(P <0.05).Compared with the HGB group,Lee index and The body weight remarkably decreased at the end of the ninth-week.2.At the end of the ninth-week,the concentration of endotoxin、TNF-α、TC and TG in blood of rats in two high-fat diet groups were much higher than in NG group(P<0.05). Compared with rats of HGB group,endotoxin、TNF-α、TC and TG concentration in HGA group were significantly lower than in HGB group.3.At the end of the ninth-week,compared with NG group,there was different degree of hepatic steatosis in high-fat group,HGA mainly- ~ +, no liver cell necrosis, fibrosis and inflammatory cell infiltration; HGB group mainly + ~ ++, no liver cell necrosis,inflammation cell infiltration and fibrosis.Conclusions:Fecal microbiota transplantation could ease endotoxemia and improve intestinal barrier function,decrease liver steatosis gradings and lipidemia.