MicroRNA-31 Transgenic Mice Model Of Spinal Cord Injury Of Repair Function Of Gastrointestinal Disorders

Objective1.By researching the gastrointestinal factors after spinal cord injury to explore the gastrointestinal’s mechanism after spinal cord injury.2.By studying micro RNA-31 transgenic mice Spinal cord injury model for the repair effect of gastrointestinal disorders, exploring the micro RNA-31 for the repair mechanism of gastrointestinal after spinal cord injury.Methods36 micro RNA-31 transgenic mices and 36 FVB mices are separately divided into control group and model group, the model of spinal cord injury is established by Impactor M-Ⅲ. Using BBB function score to evaluate the recovery of lower limbs of mice. HE staining is done to observe the changes of spinal cord tissue. The Real – time PCR is used to detected the expression of SSTR2 mRNA, Immunofluorescence is used to detected the expression of iNOS protein and SSTR2 protein.Results1. After building model group 3 d, 7 d and 14 d, spinal cord tissue is destructed,necrosised, vacuolizated, collagen fibers increase significantly.2. After buiding 14 d, compared FVB model group with control group, the expression of SSTR2 mRNA significantly increases(P ﹤ 0.05), the expression of iNOS protein and SSTR2 protein increases significantly.3. Compared miRNA-31 transgenic mice model group with control group, theexpression of SSTR2 mRNA significantly increases(P ﹤ 0.05), the expression of iNOS protein and SSTR2 protein increases significantly.4. Compared miRNA-31 transgenic mice model group with FVB model group, the expression of SSTR2 mRNA significantly decreases(P ﹤ 0.05), the expression of iNOS protein and SSTR2 protein decreased significantly.Conclusion1. The occurrence of gastrointestinal dysfunction after spinal cord injury may be related to the expression rising of SSTR2 mRNA, the expression rising of SSTR2 protein and iNOS protein.2. miRNA-31 may be related to recover for gastrointestinal disorders after spinal cord injury.