The Effect Of 18β-Glycyrrhetinic Acid On The Nasal Mucosa Epithelial Cilia In Rat Models Of Allergic Rhinitis

Part one :The Effect of 18β-glycyrrhetinic Acid on the Expression of AQP3 in Nasal Mucosa of Allergic Rhinitis RatsObjective: To investigate the effect of 18β-GA(18β-glycyrrhetinic acid) on aquaporin-3(AQP3)expression in Nasal Mucosa of AR Rats.Methods: AR models were established by ovalbumin-induction. The model rats were randomly divided into three groups after successful modeling, model group, hydrocortisone(HCS)group and GA group. Nasal mucosae were collected at 29 day and 36 day. The nasal mucosae of all animals were prepared for histochemical analysis, western blot and real-time polymerase chain reaction(RT-PCR).Results: It was revealed by immunohistochemical study that AQP3 was expressed at the plasma membranes of the epithelium, serous gland cells and mixed gland cells of nasal mucosa. Compared with the model group, GA and HCS could promote the AQP3 m RNA levels by RT-PCR that increased fold to 3.626 and 4.790(1w), respectively(P < 0.01), as well as 5.789 and 6.313(2w), respectively(P < 0.01); GA and HCS could promote the AQP3 protein levels by western blot that increased fold to 1.2819 and 1.405(1w), respectively(P < 0.05), as well as 2.3927 and 3.0655(2w), respectively(P < 0.01). These results were also confirmed by an immunohistochemical examination, which AQP3 expression was strengthen in the epithelium, serous glands and mixed glands of nasal mucosa.Conclusion: 18β-GA is effective to restrain the pathological changes of nasal mucosa epithelial damage and edema in rat models of AR, which may be associated with the increased expression of AQP3.Part two :The Effect of 18β-Sodium Glycyrrhetinic Acid on the Nasal Mucosa Epithelial Cilia in Rat Models of Allergic RhinitisObjective: To investigate 18β-sodium glycyrrhetinic acid impact on nasal mucosa epithelial cilia in rat models of allergic rhinitis(AR).Methods: AR models were established by ovalbumin-induction. Wister rats were randomly divided into groups as normal group, model group, budesonide(0.2 mg/kg) group and sodium glycyrrhetinic acid(20 mg/kg and 40 mg/kg)group after the success of AR models. Nasal septum mucosae were collected after 2 week and 4 week intervention, and the morp Hological changes of nasal mucosae were observed by electron microscope.Results: Compared with the model group: after 2 week intervention, thick mucous secretions on the top of columnar epithelium cilia in rat nasal mucosa was significantly reduced, and cilia adhesion, lodging, shedding were relieved in budesonide group and sodium glycyrrhetinic acid group, the relieve in budesonide group slightly better than that in sodium glycyrrhetinic acid group; after 4 week intervention, Cilia adhesion, lodging, shedding were completely vanished, and the cilia were ranged in regular direction in budesonide group and sodium glycyrrhetinic acid group. Cilia in sodium glycyrrhetinic acid(20 mg/kg) group was more orderly, smooth than that in budesonide group and sodium glycyrrhetinic acid group(40 mg/kg), and the condition of cilia in sodium glycyrrhetinic acid group(20 mg/kg) was similar to the normal group.Conclusion: 18β- sodium glycyrrhetinic acid is effective to restrain the pathological changes of nasal mucosa cilia in rat models of AR.