The Effect Of Silencing α-fetoprotein Expression In Hep G2 Cell On The Cellular Function

Objective:To construct lentiviral vectors with si RNA of AFP and investigate the affect of silencing AFP expression in Hep G2 in terms of proliferation,invasion and metastasis.Method:Synthetic si RNA-AFP and its negative control were transfected into Hep G2 cell by lentiviral vector means,then getting a stable cell line with AFP expression silenced by puromycin. Quantitative real-time PCR and Western blot was used for detecting the effects of interference in m RNA and protein levels. In addition, MTT assay was used to measure the proliferation of Hep G2 cell. The invasion ability changes were analyzed in Transwell and the migration of Hep G2 cell was detected by wound healing assay.Results:The results of q RT-PCR and Western blot demonstrated that the AFP expression was significantly decreased about 90% and(62.53±6.17)%,respectively both at m RNA and protein levels after transfection(P<0.05). The proliferation abilities of Hep G2 cell were apparently decreased(31.17±1.16)% by silencing AFP expression after culturing 6 days(P<0.05).The inhibition of silencing AFP could induce suppression of invasion and migration(P<0.05).Conclusion:Silencing AFP expression can effectively suppress the proliferation, invasion and metastasis process in Hep G2 cell.