X-ray Radiosensitizing Effect Of Fluorinated Graphene Quantum Dots On Tca-8113 Cells

ObjectiveThis paper investigates the X-ray radiosensitizing effect of Fluorinated Graphene Quantum Dots on human Tca-8113 cells and analyzes its radiosensitizing mechanism.MethodsTca-8113 was irradiate by X-ray.The safe concentration of F-GQDs was ascertained through MTT assay, which gives the IC50 and the cellular growth curve. The cell morphology was observed by inverted phase contrast microscope. The colony formation rate was measured via plate clone formation assay. The rate of ROS, cell cycle distribution and cell apoptosis was confirmed by flow cytometry; Western blot was used to detect the expression of cell apoptosis associated proteins.Results1)The IC50 of F-GQDs in 24 h was 8.23mg/ml.400ug/ml was selected for the safety concentration of F-GQDs(P≤0.05).2)MTT assay and plate clone formation assays demonstrate that groups intervened with F-GQDs inhibited cell proliferation significantly in different X-ray doses(P≤0.05).3)Flow cytometry shows that groups intervened with F-GQDs have higher rates of reactive oxygen species(P≤0.05), greater cell apoptosis ratio, and more cell arrested in G2/M phase in different X-ray doses.4)Western blotting assays indicate that groups intervened with F-GQDs has an up-regulated expression of Casepase-3, p53, Bax, and down-regulated Bcl-2.ConclusionThe experiments indicate that F-GQD shave a X-ray radiosensitivity effect on Tca-8113.One of underlying mechanisms could be correlated with the increase of ROS, which facilitates cell apoptosis. In addition, the cell cycle G2/M phase arrested enhance the radiosensitization of human Tca-8113 cell to X-ray. They all inhibitte the growth of Tca-8113 cell eventually.