| Objectives The research of natural plant products applied in oral medicine are more and more widely.Lemon essential oil(LEO) is the focus research of our team in the role of natural plant products in caries disease prevention and control;Limonene(LIM) as the main components of the LEO; Hesperidin(HPN) is one of the Lemon peel extract(LPE) ingredients, but in the Lemon essential oil content is very low; Tea Polyphenols(TP) is recognized as a kind of natural plant products have their effect.Hard tissue damage mainly for dental decay, lactate dehydrogenase(LDH) of Streptococcus mutans(S.mutans) using carbohydrate metabolism to produce acid, causing local p H within the plaque is reduced, cause the local demineralization of tooth enamel, caries damage of formation; In soft tissue diseases, there are a large number of literature reports, too many free radicals in the mouth can cause the occurrence of periodontal disease, and removal of local increase of oxygen free radicals can promote healing of oral ulceration promote the healing of maxillofacial injury wounds. This experiment is to contrast TP, HPN, LEO and LIM on the producing acid and lactic dehydrogenase activity, as well as to the removal ability of free radicals, further explore play a major role in lemon extract ingredients; And for the future development of natural, non-toxic side effects, a new type of ecological provides the theory basis for oral disease prevention and control of preparation.Methods1. Determining the effect of four kinds of natural products on S.mutans LDH activity Selecting S.mutans UA159 as experimental strain. Determine the minimal inhibitory concentration(MIC) of LEO, HPN, LIM, TP on S.mutans. Select three concentrations following MIC as experiment concentrations,and blank control group is BHI liquid medium without any drugs. Reducing coenzymeâ… oxidation method is used to determine the effect of them on S.mutans LDH activity.2. Determining the effect of four kinds of natural products on S.mutans produce acid metabolismFE20 laboratory pH meter is used to test the change of p H value(p H = initial p H-delta terminal p H value) before and after medication medium, to detect the inhibitionof four kinds of natural products on ability of S.mutans acid producing, and acid producing ability and activity of LDH do correlation analysis.3. The contrast study of the four kinds of natural products, remove free radical Select four natural products MIC group as experimental group, using UV- Vis spectra to analysis the removal ability of TP, HPN, LEO and LIM of 1, 1 diphenyl- 2- trinitrobenzene hydrazine(DPPH ?) and hydroxyl radical(? OH).Results1.MIC of LEO, HPN, LIM, TP were 4.5 mg/ml, 8 mg/ml, 23 mg/ml, 4 mg/ml.2.LEO,HPN,LIM,TP can inhibit LDH enzyme activity and produce acid of S.mutans, compared with the blank control group differences statistically significant(enzyme activity: FLEO = 105.186, P < 0.05; FHPN = 34.522, P < 0.05; FLIM = 31.40, P < 0.05; FTP = 28.209, P < 0.05; produce acid: FLEO = 162.05, P < 0.05; FHPN = 24.003, P < 0.05; FLIM = 73.945, P < 0.05; FTP = 32.006, P < 0.05).3.LEO, HPN, LIM, TP can clear DPPH ? and ? OH, compared with the blank control group differences statistically significant(DPPH ? radical: FLEO = 2249.965, P < 0.05; FHPN = 47.217, P < 0.05; FLIM = 21.825, P < 0.05; the FTP = 6.246, P < 0.05; ? OH radicals: FLEO = 99.977, P < 0.05; FHPN = 15.145, P < 0.05; FLIM = 9.200, P < 0.0; the FTP = 19.935, P < 0.05).Conclusion1. LEO, HPN, TP and LIM can inhibit S. mutans LDH activity and produce acid. LEO and HPN solution of S.mutans LDH activity inhibition and produce acid inhibition is a positive correlation.2. LEO, HPN, TP and LIM are able to removal DPPH ? and ? OH, and have concentration dependence. The ability to remove DPPH ? is HPN significantly better than the other three groups drugs; The ability to remove ? OH is TP significantly superior to the other three groups of drugs.3. Lemon extracts of LEO,HPN,LIM and TP can inhibit S.mutans acid production, the LDH activity of enzymes related to produce acid, and scavenging free radicals. |
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