The Relationship Between Alzheimer’s Disease And The Levels Of Folate,vitamin B₁₂ And Methionine Metabolites:a Case-control Study

ObjectiveBy measuring serum folate, vitamin B12, inflammatory factor levels and methionine cycle product level in plasma, To investigate the relationship between Alzheimer’s disease(AD) and the levels of serum folate, vitamin B12,inflammatory factors and plasma methionine metabolites, To learn more about the pathogenesis of alzheimer’s disease causes and mechanism, for alzheimer’s disease prevention and control of new ideas.MethodsEighty cases with Alzheimer’ s Disease were recruited as AD group and eighty health people were randomly selected as control group in Tianjin Huanhu hospital.In AD group,their age were 50~85 years and diagnosis with NIA- AA and DSM-IV. Their blood samples were collected to detect homocysteine(Hcy), S-adenosylmethionine(SAM) and S-adenosylhomocysteine(SAH) with high performance liquid chromatography or serum folate and vitamin B12 with chemiluminescent immunoassay. Levels of serum Interleukin-6(IL-6) 、 Tumor necrosis factor-α(TNF-α) were deteeted by ELISA.ResultsThe levels of serum folate(t = 4.730, P = 0.000), vitamin B12(t = 5.440, P = 0.000), SAM(t = 5.665, P = 0.000)and the ratio of SAM/SAH(t = 15.937, P = 0.000) in AD patients were lower than those in the health people(P<0.05). SAH(t = 2.971, P = 0.004), Hcy(t = 6.060, P = 0.000) concentration of AD patients were higher than those for the health people(P<0.05). And The raised level of folate(OR=0.863, 95%CI: 0.760,0.979), vitamin B12( OR=0.994, 95%CI:0.989,0.999), SAM(OR=0.623, 95%CI:0.478,0.813) are the independent protective factors; The level of Hcy(OR = 1.213, 95% CI: 1.046 ~ 1.405)and SAH(OR = 1.493, 95% CI: 1.163 ~ 1.917)are the independent risk factors.The levels of serum IL-6( t=7.358,P=0.000),TNF-α(t=6.304, P=0.000)in AD patients were higher than those in the health people(P<0.05). The raised level of IL-6( OR=1.117, 95%CI:1.051~1.187),TNF-α(OR=1.007, 95%CI:1.004~1.010) are the independent protective factors.ConclusionThe level of serum folate, vitamin B12,inflammatory factor and plasma methionine metabolites are related to AD. Prompt the above factors have effect on AD disease, speculated that the occurrence of the above factors may involve AD and development mechanism.