Analytical Method Of Aripiprazole In Serum By HPLC

Beside physical health, mental health plays an improtant role for people’s daily life as well as studying and working. As all kinds of mental patients increasing each year all over the world, it attracts much attention on the treatment of mental illness. Aripiprazole belongs to the third generation of atypical antipsychotics, with the characterists of little side effects, efficacy significantly, and low recurrence rate comparing to other antipsychotics. But after applying improper dose of aripiprazole some side effects, for instance, damaging the patient’s cardiovascular system, digestive system, endocrine system, blood or lymphatic system, musculoskeletal system, nervous system, respiratory system, special feeling system, urogenital system, skin and accessories will occur[1]. As a matter of fact the research on aripiprazole drug concentration detection to cooperate with the clinical treatment for mental illness, so as to avoid and reduce the side effects of drugs has huge significance. For the purpose of performance liquid chromatography, by using the aripiprazole concentration treatment for clinical, thesis provides simple rapid, sensitive and accurate drug concentration analysis method. The main research contents are as follows:1. The serum samples aripiprazole of pretreatment method, using ether extraction, nitrogen blow dry enrichment. That is more simple, easy to extract. The recovery was 78.5%~79.1%.2. The use of reverse phase chromatography using C18 chromatographic column, mobile phase respectively to compare the different solvent, methanol relative acetonitrile peak shape and separation degree is better, no tail, small toxicity. Liquid phase with the increase of organic phase ratio, peak retention samples will decrease, peak height is higher. With the decrease of the flow rate, sample peak retention will increase, peak width increases, the peak height is reduced. 25℃ and 35℃ and 45℃ three chromatogram column temperature conditions found that at 25℃ and 35℃, the peak retention time of sample is changeless, and when the temperature higher to 45℃, the peak retention time will decrease. Compared the pH4.2, 6.2 and 7.4 three of pH value, no significant change in the sample peak retention time and peak shape. Comparison of the different concentration of triethylamine to test the effect of buffer salt, the results show that triethylamine buffer salt peak retention time and peak shape of samples peak samples had no significant change. With the increase of the injection volume of sample, peak area increased. Aripiprazole and internal standard estazolam had characteristic absorption peak at 254 nm.3. Integrated the above experimental results and established the proportion of mobile phase, flow rate, column temperature as the main factors influencing the test, chromatographic conditions are optimized by the orthogonal experiment, confirmed the chromatographic column: Waters Symmetry® C18, 5 microns, 4.6 mm x 250 mm, mobile phase: water and methanol(10:90), and flow rate: 1.0 mL/min, detection wavelength: 254 nm, column temperature: 25℃, injection volume: 20 μL.4. It is verified by the final test, to determine the 25 ng/mL~800 ng/mL detection of linear range, detection limit of 10 ng/mL, compared the samples at room temperature, frozen, freezing and thawing conditions, to maintain stability. The relative recovery rate is 98.7%~ 101.2%.