The Expression Of Tumor Related Proteins In Laryngeal Carcinoma And Clinical Research

Objective: To investigate the expression of programmed death 1(PD-1), programmed death ligand 1(PD-L1) in laryngeal carcinoma and vocal cord polyp tissues, and explore their correlation to clinical pathological features of laryngeal carcinoma.Methods: The pathological specimens of 45 laryngeal carcinoma patients who received surgical treatment were collected from the department of Otolaryngology in the Affiliated Hospital of North China University of Science and Technology between 2010 and 2015, which were selected to the experimental group. The same period pathological specimens of 37 vocal cord polyp patients who received surgical treatment were collected, which were choosen to the control group. The expression of PD-1 and PD-L1 in laryngeal carcinoma and vocal cord polyp were detected by immunohistochemical mehod, and analyze the expression of PD-L1 and PD-1 compared with clinicopathologic features of laryngeal carcinoma patients.Results: 1 In the experimental group, the positive expression of PD-1 and PD-L1 in the laryngeal carcinoma tissues was 57.78%, and 66.67% respectively. PD-1、PD-L1 mainly located in cytoplasm or cell membrane of laryngeal carcinoma cells or interstitial lymphocyte. In the control group, the positive expression of PD-1 was 8.11%, and the positive expression of PD-L1 was 13.51%.The positive expression rate of PD-1、PD-L1 in experimental group is higher than that of control group, the difference between the experimental and control group was significant(P<0.01). 2 There were some correlations between PD-1 and PD-L1 was positive staining in laryngeal carcinoma. 3 The positive expression rate of PD-1 and PD-L1 in stage Ⅲ、Ⅳ laryngeal carcinoma tissues is higer than that of stage Ⅰ、Ⅱ, the difference between the two groups is significant(P<0.05). The positive expression of PD-1 in well differentiated laryngeal carcinoma tissues is higher than moderately and poorly differentiated, the difference between the theree groups was significant(P<0.01). The positive expression of PD-L1 in well differentiated laryngeal carcinoma tissues is higher than moderately and poorly differentiated, the difference between the three groups was significant(P<0.05).The age, sex of the patient and the location of tumor have no impact on the expression of PD-1、PD-L1 in laryngeal carcinoma tissues.Conclusion 1 Both PD-1 and PD-L1 protein were expressed in experimental and control groups, the difference between the two groups was significant(P<0.01). 2 The expression of PD-1 in laryngeal carcinoma was significantly correlated to the expression of PD-L1. 3 The expression of PD-1 and PD-L1 in laryngeal carcinoma was correlated to the clinical stage and the differentiate degree of tumor. By contrast, no significant difference were observed between the age, sex of the patient and the location of tumor.