The Expression Of OTUB1 In Colon Cancer And Its Relationship To DCN

Background and objective: Deubiquitinating enzymes(DUBs) play an important role in reversing theintracellular proteins, including cell cycle regulation, signal transduction, cell DNA repair, inflammatory response, tumor development and so on. Decorin is a component of extracellular matrix, which can inhibit the growth of tumor cells, reverse the phenotype of tumor cells, inhibit tumor angiogenesis, mainly through the pathway of degradation. Ovarian tumor associated proteases(OTU) belong to the members of the family of ubiquitin conjugating enzyme, which cannot only inhibit the ubiquitination of proteins, but alsopromote the ubiquitination process. The purpose of this study was to investigate the expression of OTUB1 in colon tissues, and to explore the trend of the expression of OTUB1 and DCN.Methods:Detecting the OTUB1 by immunohistochemistry in colon tissues. The expression of DCN m RNA was detected in colon cancer by RT-PCR. Selected stable transfectants otub1 colon cancer cell lines lovo and transfected with empty plasmid colon cancer cell lines(control group) as the research object, using real-time qPCR and Western blotting to detect the m RNA and protein levels of OTUB1 and DCN expression.Results:(1)Immunohistochemistry: the expression of OTUB1 in colon cancer tissue was significantly higher than that in normal mucosa, and the difference was statistically significant(P < 0.05).(2)RT-PCR results showed that the amplified 295 bp DCN bands were significantly increased, and the difference was statistically significant(P < 0.05).(3)After transfection with OTUB1 plasmid in colorectal cancer cell, the OTUB1 and DCN m RNA expression level was upregulated, and the difference was statistically significant(P < 0.05).(4)Western-blot: compared with the control group lovo cells, the stable transfection of lovo OTUB1 cells in the OTUB1 and DCN protein were significantly expressed, the difference was statistically significant(P < 0.05).Conclusion: The expression of OTUB1 protein in normal colonic mucosa, adenoma and colon cancershowed an increasing trend, and the overexpression of OTUB1 inhibited the degradation of DCN.