Expression Of CDC7 And MCM4 And The Relationship Among Them In Colorectal Cancer

Colorectal cancer(CRC) is a serious threaten to human health as a digestive tract malignant tumor, it has high morbidity and mortality in China, It is caused by the interaction of genetic and environmental factors, it is closely related to cell proliferation and signal transduction. Colorectal cancer, the same as the majority of malignant tumor, is on the basis of its disorderly development in the cell cycle cause cells to proliferate. As two kinds of proteins, the CDC7 and MCM4 is associated with the cell cycle, their all play roles in cellular DNA replication initiation period.In this study, by detecting the CDC7 and MCM4 expression in colorectal cancer and the normal colorectal tissues, the analysis of both the biological behavior of colorectal cancer(Duke’s stage, infiltration depth, differentiated degree and lymph node metastasis) relationship and the relationship between the CDC7 and MCM4, and to investigate the occurrence of colorectal cancer development role for the study of it involved in the cell cycle and the occurrence of CRC development process, recommended the clinical treatment and its prognostic indicators and provide also new targets for clinical treatment of colorectal cancer.Objective:By immunohistochemical SP method to detect CDC7 protein and MCM4 protein, RT-PCR technique to detect CDC7 m RNA and MCM4 m RNA expression in colorectal cancer and normal colorectal tissues. Analyze CDC7 and MCM4 relevant experimental data, the expression of them in colorectal cancer and study the correlation between the two in the case of colorectal cancer.Methods:Collected from January 2014 to January 2015, number of hospitals of Chengde(Chengde medical college affiliated hospital, Chengde city central hospital) surgery pathologically diagnosed 80 cases of colorectal cancer specimens, normal colorectal tissues 30 cases. Immunohistochemical labeling of CDC7 protein, MCM4 protein expression were detected in colorectal cancer and normal colorectal tissues. Using RT-PCR technique to detect colorectal cancer tissues and normal colorectal tissues. The expression levels of CDC7 m RNA and MCM4 m RNA, combined with clinical data using statistical analysis of the experimental data.Results:1 Immunohistochemisty-SP method results1.1 The expression of CDC7 protein and MCM4 protein in colorectal cancer were higher than that in normal colorectal tissues, the differences were statistically significant(P<0.05).1.2 The positive expression of CDC7 protein in colorectal cancer which was well-moderately group was significantly higher than that in low-poor group(P<0.05). The expression of CDC7 protein in Duke’s stage C was significantly higher than B stage group(P<0.05), the expression of CDC7 protein in Duke’s stage B was significantly higher than A stage group(P<0.05). The expression of CDC7 protein with lymph node metastasis was significantly higher than that without lymph node metastasis(P<0.05). The expression of CDC7 in the invasion muscular group significantly higher than the no invasion muscular group(P<0.05).1.3 The expression of MCM4 protein in colorectal cancer which was well-moderately group was significantly higher than that in low-poor group(P <0.05). The expression of MCM4 protein in Duke’s stage C was significantly higher than B stage group(P<0.05), the expression of MCM4 protein in Duke’s stage B was significantly higher than A stage group(P<0.05). The expression of MCM4 protein with lymph node metastasis was significantly higher than that without lymph node metastasis(P<0.05). The expression of MCM4 in the invasion muscular group significantly higher than the no invasion muscular group(P<0.05).The expression of CDC7 protein and MCM4 protein in colorectal cancer were not related with age and gender(P>0.05).2 By RT-PCR test results2.1 The expression of CDC7 m RNA and MCM4 m RNA in colorectal cancer were higher than that in normal colorectal tissues, the differences were statistically significant(P<0.05).2.2 The positive expression of CDC7 m RNA in colorectal cancer which was well-moderately group was significantly higher than that in low-poor group(P<0.05). The expression of CDC7 m RNA in Duke’s stage C was significantly higher than B stage group(P<0.05), the expression of CDC7 m RNA in Duke’s stage B was significantly higher than A stage group(P<0.05). The expression of CDC7 m RNA with lymph node metastasis was significantly higher than that without lymph node metastasis(P<0.05). The expression of CDC7 m RNA in the invasion muscular group significantly higher than the no invasion muscular group(P<0.05).2.3 The positive expression of MCM4 m RNA in colorectal cancer which was well-moderately group was significantly higher than that in low-poor group(P <0.05). The expression of MCM4 m RNA in Duke’s stage C was significantly higher than B stage group(P<0.05), the expression of MCM4 m RNA in Duke’s stage B was significantly higher than A stage group(P<0.05). The expression of MCM4 m RNA with lymph node metastasis was significantly higher than that without lymph node metastasis(P<0.05). The expression of MCM4 m RNA in the invasion muscular group significantly higher than the no invasion muscular group(P<0.05).The expression of CDC7 m RNA and MCM4 m RNA in cervical squamous cell carcinoma were associated with no statistically significant difference in age(P>0.05).3 The correlation analysis between CDC7 and MCM43.1 The correlation coefficient of CDC7 protein and MCM4 protein is:rs=0.368(P<0.05).3.2 The correlation coefficient of CDC7 m RNA and MCM4 m RNA is:r=0.642(P<0.05).Conclusions:1 The positive signals of CDC7 protein and MCM4 protein were significant difference between coloretcal cancer tissues and normal coloretcal mucosa, and were associated with the Duke’s stage, depth of invasion, degree of differentiation of coloretcal cancer group and lymph node metastasis. CDC7 protein and MCM4 protein have nothing to do with the factor of age and gender.2 CDC7 m RNA, MCM4 m RNA in coloretcal cancer and normal coloretcal mucosa relative expression quantitative differences were significant, and both the Duke’s stage, depth of invasion, degree of differentiation and lymph node metastasis related of coloretcal cancer. CDC7 m RNA and MCM4 m RNA have nothing to do with the factor of age and gender.3 CDC7 protein and MCM4 protein in coloretcal cancer were positively correlated. CDC7 m RNA and MCM4 m RNA in coloretcal cancer were positively correlated. The process of occur and the development was positive synergy in coloretcal cancer.