| Along with the economic development, social progress, China has made significantly progress in medical technology. However, the incidence of cancer is increasing every year, especially pancreatic cancer, which is one of the most common malignant tumors of the digestive system; due to the development and subtle of the symptoms, the prognosis is always be overlooked. Meanwhile, in China the ratio of increasing diabetes is also changing every year; according to statistics, the incidence of diabetes in 2007 was 9.7%, but, comparing to recent years was 11.6%, which is according to the latest diagnostic criteria for diabetes. Many studies show that there is a remarkable correlation between diabetes and pancreatic cancer, but it’s not definitely certain; one view consider that diabetes may be a risk factor for pancreatic cancer, while others suggested that diabetes may be one of the first clinical manifestation of pancreatic cancer. If it is through scientific research that we can separate the early stage of diabetes from pancreatic cancer, which to pancreatic cancer patients with diabetes mellitus at the same time can monitor and control the blood glucose within a reasonable security range, improving and enhancing early diagnosis of pancreatic cancer must be a remarkable step forward. This study retrospectively analyzed 89 cases of pancreatic cancer patients’ clinical data, and preliminarily explored the relationship between type 2 diabetes and pancreatic cancer, in order to improve the early diagnosis of pancreatic cancer; by culturing pancreatic cancer cell Bxpc-3 to find out the effection of different concentrations of insulin and glucose on pancreatic cancer cell on proliferation and apoptosis, we can have a guidance to clinical treatment options of controlling blood glucose targets, insulin and other drugs. Part 1 Analysis of related factors of pancreatic cancer research and new-onset diabetesObjective:To investigate the risk factors for pancreatic carcinoma(PC) and the relationship between PC and new-found diabetes mellitus(DM).Methods:We select between January 2011 to September 2014 89 cases of pancreatic cancer in Affiliated Hospital of Hebei University of Engineering as PC group, of which the average age is 63±13.2 years. While at the same period, 123 cases as the control group is non-neoplastic and non-digestive, non-metabolic abnormalities and the two groups match with age and sex. Applying of the case-control study, we can compare the clinical characteristics between PC and DM,and anaylize the characteristics of diabetes prevalence and progression by laboratory tests.Results:Smoking, DM, chronic pancreatitis in PC group was higher than DM group, the difference was statistically significant(P<0.05),but the drinking in both groups showed no statistically significant(P>0.05);Comparing to the DM group,the difference of WBC, TP, T-CHO, HDL, LDL in PC group was not statistically significant(P>0.05), while the HGB in PC group was significantly lower than the control group, and the ALP, GGT, TBIL, TG, FBG(fasting blood-glucose),HbAlc,were significantly higher than the control group, the difference was statistically significant(P<0.05);The prevalence of diabetes in the PC group comparing to 24.39% of control group was 40.44%, this difference was statistically significant(P<0.05). PC group with diabetes was 29 cases(32.58%) in duration of diabetes is less than two years, comparing to control group of123 patients with 15 cases(12.19%) within 2 years was statistically significant(P<0.05).Conclusion:Some risky factors for PC are Smoking, chronic pancreatitis, high cholesterol and abnormal liver function and DM may be associated with symptoms of PC. The Older who is over 60 year-old should be vigilant about emerging glucose fluctuations to in case of PC. Part 2 Different concentrations of glucose, insulin levels on apoptosis and proliferation of pancreatic cancer cell Bxpc-3Objective:Through simulation of type 2 diabetes in the course of the change of different concentration of glucose, insulin, we can study its proliferation apoptosis of pancreatic cancer cell lines Bxpc-3 and discuss the possible mechanism. All these efforts are to clarify the early stage of pancreatic cancer patients with different blood glucose, insulin levels of reproduction and apoptosis of pancreatic cancer, providing a theoretical basis for the control of blood sugar and how to use insulin treatment and insulin dosage.Methods:First, we culturing pancreatic cancer cells in vitro, then we choose experimental group by providing various concentrations of glucose, insulin, and the control group is without glucose and insulin. Application of MTT and flow cytometry to detect the effection of proliferation on human pancreatic cancer cell line Bxpc-3 with different concentrations of glucose, insulin, glucose with insulin. Results:The difference that the different concentration of normal glucose group and high glucose group on Bxpc-3 proliferation is statistically significant(P <0.05).But comparing the normal glucose group with the control group, the difference was not statistically significant(P>0.05), the difference of high glucose compared to the control group is statistically significant(P<0.05). The results show that high glucose has great effect on human pancreatic cancer cell Bxpc-3 proliferation. Difference that the different concentration of normal insulin group and high insulin group on Bxpc-3 proliferation is statistically significant(P<0.05).But comparing the normal insulin group with the control group, the difference was not statistically significant(P>0.05), the difference of high insulin compared to the control group is statistically significant(P <0.05). The results show that high insulin has great effect on human pancreatic cancer cell Bxpc-3 proliferation. Glucose groups with insulin do not show the synergy on human pancreatic cancer cell line Bxpc-3 proliferation, which G group(normal glucose + high insulin group) cell proliferation compared with F group(normal glucose + normal hormone insulin group), H group(high glucose + high insulin group), I group(normal high glucose plus insulin group) is stronger(0.671±0.014), and the difference was statistically significant(P <0.05). The difference between normal glucose group and high glucose group with different concentration on pancreatic cancer cell Bxpc-3 apoptosis is statistically significant(P<0.05), but the normal group comparing to the control group had no statistically significant(P>0.05),while the difference of high glucose group comparing to the control group is statistically significant(P<0.05). These results show high glucose can decrease the apoptosis in human pancreatic cancer cell line Bxpc-3. Difference between normal insulin group and high insulin group with different concentration on pancreatic cancer cell Bxpc-3 apoptosis is statistically significant(P<0.05), but the normal group comparing to the control group had no statistically significant(P> 0.05),while the difference of high insulin group comparing to the control group is statistically significant(P <0.05). These results show high insulin can decrease the apoptosis in human pancreatic cancer cell line Bxpc-3. Glucose groups with insulin do not show the synergy on human pancreatic cancer cell line Bxpc-3 proliferation, which G group(normal glucose + high insulin group) cell proliferation compared with F group(normal glucose + normal hormone insulin group), H group(high glucose + high insulin group), I group(normal high glucose plus insulin group) is weaker(8.5±0.8), and the difference was statistically significant(P<0.05).These results show the higher concentration of glucose and insulin can decrease the ratio of apoptosis on Bxpc-3,which the insulin is remarkable,but the two aspects have no synergy. All these results show that glucose and insulin bring into correspondence with the proliferation and apoptosis of Bxpc-3.Conclusion:Both high concentration of glucose and insulin can increase the proliferation capacity, decrease apoptosis rate and depress the apoptpsis, but there is no synergy between the two. |
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