The Research Of Anti-Parasitic Of Tradition Chinese Herbal Compound C

Objective:Screening of traditional Chinese Medicine Anti parasites(compound C), we developed the C compound, and provide a reference for the research and development of anti parasite drugs.Method:①Animal model of animal I as host and parasite Y was set up, and the effectiveness of the method was established by using the model, and the insecticidal activity of the extracts of the 19 traditional Chinese medicine extracts was screened.②The chemical evaluation method by UV Spectrophotometry of compound C, compound C extraction method, solvent selection, crushing degree, solvent dosage, extraction time and extraction times of these single factor were investigated. The optimized level of each single factor, using 4 factors and 3 levels of L9(34) orthogonal experimental design optimization the best extraction process of compound C, combined with the comprehensive evaluation of production factors demand optimal preparation technology of compound extract of C.③According to the safety test on the test animal C compound with different concentration, the average mortality rate of experimental animal into probability unit, using the method of Bliss calculation and analysis of experimental animal 24, 96 h 48, the semi lethal concentration of LC50, the Probit probability unit in SPSS13.0 software for statistical analysis.④Dichloromethane methanol formic acid(93:9:4) as the agent, the referencesolution sample quantity is 1L, the testsolution sample volume 5 L, chromogenic agent: phosphomolybdic acid(10%), a qualitative identification of C compound by tlc.⑤The following conditions:mobile phase: methanol-0.4% phosphoric acid(27:73), Eclipse Plus C18 column:Agilent(4.6 x 250, 5 m), column temperature: 30 degrees centigrade, the detection wavelength: 323 nm, detector: DAD, analysis of time: 25 min, flow rate: 1ml/min, high performance liquid chromatography method for determination the C compound.Result:Screening out the best prescription of traditional Chinese medicine C anti Y effect of animal parasites. The maximum UV absorption wavelength of C compound is 323 nm, linearity, repeatability, stability and recovery. The methodology is less than2%. C compound preparation process: crushed through 60 mesh sieve, selection of solvent IV, reflux extraction 3 times, each time 30 min, the amount of solvent extraction were 8 times, 8 times and 6 times.C compound at the dose of 20mg/L,killing for Y of parasite rate could reach 100%, 96 h test animal lethal concentration was 1566.3mg/L. Safe and effective herbal C extract against animal parasites of Y,provide the basis for the development and application of new traditional Chinese Medicine Anti parasite Y.Conclusion:Safe and effective herbal C extract against animal parasites of Y, provide the basis for the development and application of new traditional Chinese Medicine Anti parasite Y.