Effect Of TWEAK On LOX-1 Expression In Human Umbilical Vein Endothelial Cells

Objective:To investigate the effect of tumor necrosis factor-like weak inducer of apoptosis in human umbilical vein endothelial cells(HUVECs) on lectin-like oxidized low-density lipoprotein receptor-1(LOX-1) expression.Methods:Cultured HUVECs by 0,10,50,100 ng/ml of recombinant human TWEAK(rh TWEAK) intervention 24 h, the relative expression using quantitative PCR to detect different concentrations of TWEAK amount of HUVECs cells LOX-1 m RNA, and 0.2,1,10 μmol/l atorvastatin intervention 24 h express PT-PCR to detect cells LOX-1 m RNA of HUVECs cells after 24 h use 100ng/ml TWEAK incubation, while each group was detected by ELISA in cell supernatants LOX-1 protein expression.Results:Different concentrations of TWEAK intervention group LOX-1 m RNA 2- △△ Ctvalues were 0.883 ± 0.195,1.123 ± 0.275,1.610 ± 0.291,2.180 ± 0.437, TWEAK intervention group supernatants LOX-1 protein expression levels were relatively 0.758 ±0.071,1.128 ± 0.010,1.468 ± 0.122,1.793 ± 0.118 ng/ml, TWEAK increases with increasing concentrations of LOX-1 expression levels, compared with the control group and the difference between groups was statistically significant(P<0.001);atorvastatin intervention group LOX-1 m RNA 2- △△ Ctvalue of 1.430±0.328,1.236±0.248,1.091±0.218, cell supernatants relative expression of LOX-1 protein in an amount of 1.296±0.152,1.145±0.039,1.006±0.062 ng/ml, different concentrations of atorvastatin after the intervention significantly inhibited the expression of LOX-1m RNA and protein, the difference between groups was statistically significant(P<0.001).Conclusion:Different concentrations of TWEAK can induce human umbilical vein endothelial cells upregulate the expression of LOX-1, which may have caused to its role of atherosclerosis; Atorvastatin inhibits LOX-1 expression, may be one of the mechanisms to play the anti-inflammatory and anti-artery atherosclerosis role.