| Objective:TO establish the rat model of interstitial cystitis,explore the relationship between mast cells and PAR-2 by the expression of mast cells and PAR-2,seek new targets for the IC.Methods:35 SD female rats were randomly divided into 3 groups. Experimental group 10 (modeling group) 1,Experimental group 10(PAR-2 inhibitor treatment immediately after the establishment of the model) 2,Control group of 10 (phosphoric acid buffer solution group). (1) The experimental group of 1 transurethral intravesical instillation of 10 mg/ml 1 ml protamine sulfate, keep 45 min,wash the three times with PBS after discharg; The lipopolysaccharide of 750ug/ml was 1 ml,keep 30 min,wash the three times with PBS after discharg, pull out the catheter.Injection 1 times in 2 days,4 weeks of injection. (2) Experimental group 2 on the basis of the experimental group lto join the bright anti 200mmol/l, keep 15-30min, Injection 1 times in 2 days,4 weeks of injection. (3) Control group of bladder perfusion PBS.1. Pathological characteristics of IC animal model were observed by HE staining;2. the number of mast cells in bladder of rats were detected by toluidine blue staining;3. The number of PAR-2 positive cells and MC positive cells was calculated by immunohistochemistry staining;4. PT-PCR calculation of the experimental object PAR-2 expression;5. Masson staining was used to observe the changes of collagen fibers and muscle fibers in IC animal model.Results:1. Bladder mucosa thinning in the experimental group and in the lamina propria capillary dilatation and congestion, in the muscular layer of the fibrous tissue hyperplasia, infiltration with lymphocytes and mast cells; control group, the bladder mucosa thinning, natural capillary layer without expansion, inflammatory cells are few and muscular layer of fibrous tissue proliferation is not obvious.2. Mast cells by toluidine blue stained purple red, significantly increased the number of mast cells in experimental group 1, especially removing mast cell granules; experimental group 2, rare cell degranulation; control group saw only mild increase in mast cells。3. PAR-2 protein was brown in positive reaction product expression in tissues of the experimental group,1 in the visible positive cells were brown yellow; while the experimental group 2, control group almost impossible to find similar positive cells。 4. In the experimental group, 1Masson staining showed that the proliferation of collagen fibers and muscle fibers;The experimental groiup 2 and control group rare hyperplasia of collagen fiber.Conclusion(s):1. This experiment by perfusion protamine and lipopolysaccharide successful build IC model.2. In the experimental group, the expression of PAR-2 increased, the treatment of the inhibitor, the bladder fibrosis was significantly improved, indicating that PAR-2 was positively correlated with the interstitial cystitis.3. In the experimental group, the mast cells of the bladder tissue increased significantly, and the granule cells were significantly increased, and the bladder fibrosis was obvious. Therefore, there was a positive correlation between the mast cells and interstitial cystitisAMC and PAR-2 play an important role in IC bladder tissue fibrosis; inhibiting the expression of PAR-2 can reduce the IC of bladder tissue fibrosis... |
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