Study In Chemical Component Changes And RCT Effects Of Radix Bupleuri And Its TCM Compatibility

ObjectiveFirst, research to the variation of saikosaponins from radix bupleuri and its TCM compatibility in decocting; second, research to Saikosaponin variation in metabolizing and the differences of metabolites; Third, research to how radix bupleuri and its TCM compatibility impact the reverse cholesterol transport. Try to explain scientific of radix bupleuri and its TCM compatibility from chemistry characterization, and provide scientific reference for basic research material of radix bupleuri efficacy.Methods1. To choose Radix Bupleuri combine with Radix et Rhizoma Rhei, Radix Paeoniae Alba, cassia twig, Scutellaria and oyster as the research object, established of corresponding feature map, and tested extraction process of stability, and contented determination of saikosaponin a, saikosaponin d, saikosaponin b1, saikosaponin b2 by HPLC.2. Using HPLC-DAD quantitative analysis of saikosaponin components in the metabolic process whether receives the influence from other components and medicine in decocting, and using LC-MS qualitative analysis their chemical composition metabolize before and after.3. Using the model of HepG2 cells uptake DiI-HDL to observe the effect from radix bupleuri and its TCM compatibility metabolize before and after.Results1. Compared with the single decoction of Bupleurum group, saikosaponin a in 1:1 and 2:1 Radix Bupleuri combine with Radix et Rhizoma Rhei group decreased and saikosaponin d, saikosaponin bi, saikosaponin b2 increased. Only saikosaponin a in 1:1 and 8:3 Radix Bupleuri combine with Radix Paeoniae Alba group increased. Saikosaponin a and b2 in 1:1 Radix Bupleuri combine with cassia twig group decreased and saikosaponin d and b1 increased; but all kinds of saikosaponin in 8:3 group increased. All kinds of saikosaponin in 1:1 and 8:3 Radix Bupleuri combine with Scutellaria group increased. All kinds of saikosaponin in 8:3 Radix Bupleuri combine with oyster group increased, but in 1:1 Radix Bupleuri combine with oyster group only saikosaponin a and d increased2. In saikosaponin group and the single decoction of Bupleurum group, saikosaponin a changed less obvious, saikosaponin d was decreased first and then increased to reduce and increase trend, saikosaponin bi showed increasing trend after a decreasing trend, saikosaponin b2 was decreased and in 12 hours after fell to 0. In Bupleurum combine with Scutellaria group, saikosaponin a increased obvious, saikosaponin d and b2 showed a decreasing trend after increased but then increased, saikosaponin bi changed little. In Radix Bupleuri combine with oyster group, saikosaponin a changed less obvious, saikosaponin d decreased first and then increased, and saikosaponin b1 decreased first and then increased but changed not obcious, saikosaponin b2 was decreased but did not fell to 0.3. Seven prototypes were detected:saikosaponin a, saikosaponin d, saikosaponin b1, saikosaponin b2,4"-O-acetylsaikosaponin d, saikosaponin f, saikosaponin i and C48H78O17 in t=38.6; five metabolites:prosaikogenin F, prosaikogenin D, prosaikogenin A, prosaikogenin G, and C36H58O8 in t=19.9. Metabolites were only detected in 24h of Bupleurum and 24h of Bupleurum combine with oyster.4. The ability of the HepG2 cells to absorb HDL was increased by Bupleurum and Bupleurum combine with oyster whether metabolize before or after, and the effects from Bupleurum combine with oyster metabolize before or after were the most significant. Bupleurum combine with Scutellaria metabolize before promoted to absorb HDL, but metabolize after inhibited.ConlusionWhen bupleurum combine with other medicine, the solubility of saikosaponin increased, and when TCM compatibility comes from "Shang Han Lun" and "Jingui Yaolue", the solubility of saikosaponin increased observably. It meant radix bupleuri and its TCM compatibility was scientific. The flavonoids did not affect saikosaponin in metabolism. Hydrolysis and transformation were characterizations of Radix Bupleuri and Bupleurum combine with oyster metabolizing, and only transformation in Bupleurum combines with Scutellaria. After metabolism, Radix Bupleuri reduced toxicity and had stronger ability to absorb HDL. The effects from Bupleurum combine with oyster metabolize before or after were the most significant. But Bupleurum combine with Scutellaria metabolize after inhibited absorb HDL. The differences in medicine efficacy between the products before and after metabolism may be related to different types of metabolism.