Continuous Passive Motion Had An Influence On Elastic Protein And Type Ⅰ Collagen Protein Expression In Deputy Ligament After Rabbits’ Knee Injury

Objective By observing the expression of type I collagen and elastic protein the lateral collateral ligament in, to explore the effect of Continuous passive motion on joint damage early from the molecular level, to provide the theory basis of molecular level for Continuous passive motion to promote joint rehabilitation,had the vital significance for using continuous passive motion(Continuous passive motion, CPM) treatment largely and reasonably.Methods15 male New Zealand white rabbit was randomly divided into 3 groups: brake group,free activities group, continuous passive motion group,which age was 70 days, weight was 1.165 ~ 2.335 kg.Used 3% pentobarbital sodium(1 ml/kg) for anesthesia in the ear marginal vein, made a 2 cm curved incision in the inside of the knee patella,layered cut to visible joint capsule and exposed femoral patellar surface, with the femoral medial condyle as a center, used orthopaedic surgical drill that diameter was3.2 mm size grinding to establish full-thickness cartilage defect, there were a few ooze blood shall prevail in the defect area, as shown in figure 1.Used 250 ml saline repeatedly to wash coagulate blood clot and the remaining pieces of the operation of cartilage until washed clean. Closed the incision with suture line 4 in order, and dressed incision with sterile gauze and medical bandage properly.Rest 1 day postoperative in cage, brake group: used homemade leg warmers for braking; Free activity group: only free activities in cage; Continuous passive motion groups:continuous passive motion 2 hours every day(morning, afternoon 1 hour), a total of six weeks. There is not brake after continuous passive motion, all the rabbit feeding in cage separately. Collected ligament and extracted total protein after six weeks, used western blot to for testing the expression of elastin and type Ⅰ collagen protein in brake group, free activity group, CPM group, and scan imaging. Determined the samples electrophoresis banding grey value of β—actin,elastin and type Ⅰcollagen protein, the β—actin electrophoresis banding as reference, calculate the sample type Ⅰ collagen protein electrophoresis banding grey value and β—actin electrophoresis banding grey value ratio and elastic protein electrophoresis banding grey value and beta actin electrophoresis banding grayscale value ratio.f Used the single factor analysis of variance(One- Way ANOVA) statistical methods to solve the obtained data with SPSS16.0 system such as statistical analysis software package.All of the rabbit knee appear different degree of stiffness in brake group one knee appeared necrosis and postoperative 6 weeks the weight of all rabbits reduced than preoperative in brake group. Elastin and type Ⅰ collagen protein expression of the lateral collateral ligament in free activities group were higher than braking and CPM group there are differences in the results, there is statistical significance(P < 0.05),elastin and type Ⅰcollagen protein expression of the lateral collateral ligament in the CPM group was higher than the brake group, there are differences in the results, with statistical significance(P < 0.05).ResultsConclusions1. the activity of continuous passive motion and free activity can repair effectively after joint damaging l, which can effectively prevent ligament contracture and joint stiffness which caused by it.2. curative effect of continuous passive motion and free activities, compared to free the effect is better.